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pEF-mRuby3

Plasmid

#179730

Purpose

Mammalian expression of mRuby3 from the EF1 promoter

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

mRuby3

Expression

Mammalian

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pOCC82

Plasmid

#118862

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant protein

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-stop-HindIII cassette

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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C232-E05: attB2r-meGFP-attB3

Plasmid

#162902

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric enhanced Green fluorescent protein

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔRRM1+2

Plasmid

#35267

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 298-429

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
Myc-PSFΔN

Plasmid

#35184

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

N-terminal deletion of residues 9-264

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
Ubiquitin I44A

Plasmid

#12648

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

Ubiquitin (UBC Human)

Expression

Bacterial

Mutation

Ile44Ala

Available Since

July 31, 2007

Availability

Academic Institutions and Nonprofits only
p414GPD-3xFLAG-GW

Plasmid

#63780

Purpose

yeast expression vector p414GPD modified such that it is Gateway converted and introduces a 3xFLAG tag at the N-terminus of the insert

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Type

Empty backbone

Tags

3xFLAG

Expression

Yeast

Promoter

GPD

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDest-623 (attR4-attR3)

Plasmid

#161878

Purpose

Multisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔRRM2

Plasmid

#35377

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 375-429

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
C413-E15: attB4-EF1p>-attB1r

Plasmid

#162920

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human EF1alpha promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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