We narrowed to 12,975 results for: lic

1. pLX304_CMV::mCherry-LgBiT-FLAG-NES

   Plasmid

   #199715

   Purpose

   Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   mCherry-LgBiT-FLAG-NES
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gq)

   Plasmid

   #230020

   Purpose

   AAV vector expressing anti-mCherry PAGER(Gq) in neurons

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-LaM6-TEVcs-ALFA-hM4D
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDAS12395_PEAR-GFP_2.0-preedited

   Plasmid

   #177185

   Purpose

   PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. V5-miniTurbo-NES_pCDNA3

   Plasmid

   #107170

   Purpose

   expresses V5-tagged miniTurbo in the mammalian cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Expression

   Mammalian

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMP11

   Plasmid

   #215559

   Purpose

   pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   Cas9
   gRNA targeting pBR322 origin of replication
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23105 and tet

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ2259 - Intron GG2 - 900 bp

   Plasmid

   #159881

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG2 - 900 bp
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ2215 - Intron GG3 - 900 bp

   Plasmid

   #159882

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG3 - 900 bp
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ2214 - Intron GG1 - 900 bp

   Plasmid

   #159880

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG1 - 900 bp
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

   Plasmid

   #209725

   Purpose

   Expresses cytosolic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CamKII

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGWB402SC

    Plasmid

    #224552

    Purpose

    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.

    Depositor

    Paul Schulze-Lefert

    Article

    Lawson et al bioRxiv 2024.07.14.603419

    Type

    Empty backbone

    Tags

    Twin-Strep-HA

    Expression

    Plant

    Promoter

    2 X 35S

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB402SN

    Plasmid

    #224551

    Purpose

    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

    Depositor

    Paul Schulze-Lefert

    Article

    Lawson et al bioRxiv 2024.07.14.603419

    Type

    Empty backbone

    Tags

    HA-Twin-Strep

    Expression

    Plant

    Promoter

    2 X 35S

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TurboID-V5_pRS415

    Plasmid

    #107167

    Purpose

    expresses V5-tagged TurboID in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Yeast

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-GFP-TurboID-NES_pLX304

    Plasmid

    #202019

    Purpose

    expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    Flag-GFP-TurboID-NES
    

    Use

    Lentiviral

    Tags

    Flag and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDAI-SceI-SacB

    Plasmid

    #113635

    Purpose

    Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

    Depositor

    Miguel Valvano

    Article

    Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

    Insert

    sacB
    

    Expression

    Bacterial

    Mutation

    Please see Depositor Comments

    Promoter

    sacB promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(Gq)

    Plasmid

    #230015

    Purpose

    Expresses anti-TNFalpha PAGER(Gq) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-Ozoralizumab-TEVcs-ALFA-hM1D
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    W442A on hM1D

    Promoter

    CMV

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3

    Plasmid

    #209727

    Purpose

    Expresses cytosolic medium affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95K.miRFP670nano3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSB-CMV-MCS-Puro cPLA2-EGFP

    Plasmid

    #162568

    Purpose

    Used as a measure of cPLA2 activation in live cells with an EGFP tag.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Insert

    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LD-B

    Plasmid

    #209861

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. C1(1-29)-TurboID-V5_pLX304

    Plasmid

    #107175

    Purpose

    expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Lentiviral

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRBC-sfGFP-134/150TAG

    Plasmid

    #174077

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoter

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    D134TAG + N150TAG

    Promoter

    T7

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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