165,307 results

1. pAAV-Ef1a-fDIO mCherry

   Plasmid

   #114471

   Purpose

   Flp-dependent mCherry Control

   Depositor

   Karl Deisseroth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Promoter

   Ef1a

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. rGluA2 (px330:2-guideCas9)

   Plasmid

   #169439

   Purpose

   Expression of Cas9 and 2 guides

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   spCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 12XCSL-d1EGFP

   Plasmid

   #47684

   Purpose

   Notch reporter

   Depositor

   Urban Lendahl

   Article

   Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

   Insert

   TP-1 promoter
   

   Use

   Notch reporter construct

   Tags

   d1EGFP

   Mutation

   hexamerized 50-bp EBNA2 response element of the T…

   Promoter

   NA

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_hSyn1_AchLightG

   Plasmid

   #187464

   Purpose

   Expresses green acetylcholine indicator AchLightG in neuronal cells

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Insert

   AchLightG
   

   Use

   AAV

   Tags

   Igκ leader

   Promoter

   human Synapsin-1

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

   Plasmid

   #50464

   Purpose

   Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote

   Depositor

   Bryan Roth

   Insert

   hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. ActTS-GR

   Plasmid

   #79774

   Purpose

   FRET-based actinin tension sensors

   Depositor

   Tetsuya Kitaguchi

   Article

   Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

   Insert

   ActTS-GR
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR_DKO

   Plasmid

   #183192

   Purpose

   Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

   Depositor

   Lang Li

   Article

   Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6, mU6

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRM210 LGP (Luciferase GFP Puro)

   Plasmid

   #174722

   Purpose

   Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   eGFP
   pac
   

   Use

   Lentiviral and Luciferase

   Tags

   E2A linker and F2A linker

   Expression

   Mammalian

   Promoter

   MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG.PKCepsilon

   Plasmid

   #10795

   Depositor

   Alex Toker

   Article

   Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

   Insert

   PKC epsilon (PRKCE Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD-MSP1D1

    Plasmid

    #161607

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    D-MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1; all tryptophans were replaced b…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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