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SG-C-Int-15

Plasmid

#117044

Purpose

Constitutive integrase cassette - Bxb1-Tp901-Int5-Int7

Depositor

Jerome Bonnet

Publication

Guiziou et al Nat Commun. 2019 Jan 28;10(1):456. doi:

Insert

Constitutive integrase cassette - Bxb1-Tp901-Int5-Int7

Use

Synthetic Biology

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1104

Plasmid

#119248

Purpose

knockdown pqsC in P. aeruginosa

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA pqsC (P. aeruginosa)

Expression

Bacterial

Mutation

D10A and H840A

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1102

Plasmid

#119246

Purpose

knockdown phzA1 in P. aeruginosa

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA phzA1 (P. aeruginosa)

Expression

Bacterial

Mutation

D10A and H840A

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1103

Plasmid

#119247

Purpose

knockdown phzM in P. aeruginosa

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA phzM (P. aeruginosa)

Expression

Bacterial

Mutation

D10A and H840A

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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peSpCas9-J23109

Plasmid

#113152

Purpose

Plasmid constitutively expressing eSpCas9 protein

Depositor

Chong Zhang

Publication

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

eSpCas9

Expression

Bacterial

Mutation

K810A & K1003A & R1060A

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-HISx6-SUMOx10

Plasmid

#116937

Purpose

Bacterial expression vector for 10 sumo

Depositor

Liam Holt

Publication

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10 SUMO

Tags

HIS

Expression

Bacterial

Promoter

T7

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-Sa sgRNA expression vector

Plasmid

#107720

Purpose

For in vitro transcription of Sa sgRNA from the T7 promoter.

Depositor

Xingxu Huang

Publication

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRB DRT7VTPLux*500

Plasmid

#107959

Purpose

Controller plasmid [ca. 558.5 relative RBS strength at PLas81*]

Depositor

Jim Haseloff

Publication

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

T7RNAP (modified)

Use

Synthetic Biology

Expression

Bacterial

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_C4300

Plasmid

#91103

Purpose

Module C, Promoter: none, Gene: BaeI LacZ cassette for GT donor cloning, Terminator: none

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

BaeI LacZ cassette for GT donor cloning

Use

Unspecified

Promoter

none

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0701

Plasmid

#91019

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_H840A nickase

Use

CRISPR

Mutation

H840A

Promoter

35S

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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mKO2-Rab5a-7

Plasmid

#57892

Purpose

Localization: Ras GTPase, Excitation: 551, Emission: 565

Depositor

Michael Davidson

Insert

Rab5a

Tags

mKO2

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
mEos-A69V-N1

Plasmid

#54754

Purpose

Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Type

Empty backbone

Tags

mEos A69V, V123T, T158H

Expression

Mammalian

Available since

June 11, 2014

Availability

Industry, Academic Institutions, and Nonprofits
rME

Plasmid

#32613

Depositor

Connie Cepko

Publication

Billings et al PLoS One. 2010 . 5(10):e13739.

Insert

rME

Expression

Mammalian

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pIC-RFP-1

Plasmid

#254928

Purpose

All-in-one plasmid developed for inducible CRISPRi-assisted genome editing in Streptomyces

Depositor

Gilles van Wezel

Publication

Bai et al Nucleic Acids Res. 2026 Jun 8;54(11):gka

Insert

dCas9

Use

CRISPR and Synthetic Biology

Promoter

SP30

Available since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pTRE3G-LATeNT

Plasmid

#240988

Purpose

Dox-inducible expression of LATeNT in the mammalian cytosol; lentiviral vector

Depositor

Alice Ting

Publication

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

V5-LATeNT

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSC101-MP-mRFP1

Plasmid

#240204

Purpose

Low copy mRFP1 reporter for monitoring metabolite-responsive CRISPRa activity

Depositor

Wilfred Chen

Publication

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

mRFP1

Expression

Bacterial

Promoter

J3-DA9-UP1-MP1

Available since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pJL296

Plasmid

#243700

Purpose

A piggyBac vector containing a synthetic reporter with mNeonGreen2(11)

Depositor

Jonathan Weissman

Publication

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

mNeonGreen2(11)

Expression

Mammalian

Available since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-DARPin-SpyCatcher

Plasmid

#243754

Purpose

For affinity purification of E01 anti-EGFR DARPin fused to SpyCatcher

Depositor

Feng Zhang

Publication

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

DARPin-SpyCatcher

Expression

Bacterial

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCascade_SPS1(PmcCAST)

Plasmid

#224926

Purpose

Expression and purification of type I-B2 Cascade in CRISPR-associated transposon of Romsetta

Depositor

Leifu Chang

Publication

Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

Inserts

Cas5, Cas6, Cas7, Cas8, Cas11
CRISPR assay for crRNA expression

Use

CRISPR

Expression

Bacterial

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_Melt-37-mCherry

Plasmid

#234060

Purpose

Encodes a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Publication

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

Melt-37-mCh

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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