We narrowed to 65,871 results for: des.1
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Plasmid#173934PurposeBacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn3-L713F (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 214-373; L713F, V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G871L
Plasmid#174798PurposeBacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A870L
Plasmid#174797PurposeBacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-K893I
Plasmid#174799PurposeBacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ABI2-1/1-SV1/2
Plasmid#91285PurposeProtein expression and purification of human SH3 domain construct ABI2-1/1-SV1/2DepositorInsertABI2-1/1-SV1/2 (ABI2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVZ_PcoaT_SQE1_CAS1
Plasmid#103856PurposeHeterologous, cobalt-inducible expression of SQE1 and CAS1 from A. thaliana in Synechocystis sp. PCC6803DepositorInsertcycloartenol synthase 1 (CAS1 Mustard Weed)
UseSynthetic BiologyExpressionBacterialMutationinserted genes are codon optimized. 2nd amino aci…PromoterPcoaT (from Synechocystis sp. PCC 6803)Available SinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
AP1298‐1
Plasmid#87248Purposegtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)DepositorInsertgtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(471-660)
Plasmid#51766Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
TagsT7ExpressionMammalianMutationdeleted 1-470Available SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7Jip1(282-470)
Plasmid#51703Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
TagsT7ExpressionMammalianMutationdeleted 1-281 and 471-660Available SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pLeGO.sgGata1.4.RUNX1A.iG2
Plasmid#181976Purposegene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)DepositorAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-CMVd1-NLS-GAL4-iLight2-VP16
Plasmid#219550PurposeNLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.DepositorInsertNLS-Gal4(1-65)-iLight2-VP16
UseAAVExpressionMammalianMutationThe (SAGG)x4 linker between GAL4(1-65) and the PC…PromoterCMVd1Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
tetO-FOSL1
Plasmid#170687Purposedoxycycline-inducible overexpression of FOSL1DepositorInsertFOSL1 (FOSL1 Human)
UseLentiviral; Doxycycline inducibleExpressionMammalianPromoterTRE promoter, Tet-ONAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3039
Plasmid#144515PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-VARNAM2-Kv2.1PR
Plasmid#195524PurposeRed fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAVExpressionMammalianMutationVARNAM SI linkerPromoterSynapsinAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-pAceR-Kv2.1PR
Plasmid#195525PurposeRed fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAVExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterSynapsinAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-Squash-SAM-sensor 5-1
Plasmid#177917PurposeExpresses Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Squash-SAM-sensor 5-1
ExpressionMammalianPromoterU6+27Available SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1
Plasmid#158758PurposeCell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).DepositorInsertnullCoChR-GCaMP6f-Kv2.1
UseAAVExpressionMammalianPromotersynapsin promoterAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only