We narrowed to 2,153 results for: tev cleavage
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Plasmid#28154PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPHD finger protein 13 (PHF13 Human)
TagsHis tag with TEV cleavageExpressionBacterialMutationPHD-type zinc finger domain (residues 232-281).Available SinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-RBP-GFP(S45C)-ZdK1
Plasmid#185791PurposeEncodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linkerDepositorInsertRBP-sfGFP(S45C)-ZdK1
Tags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationSer to Cys mutation at start of GFP, residue 45 i…PromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEG608
Plasmid#40194DepositorInsertMex-5 (RNAi resistant) (mex-5 Nematode)
Tags3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…ExpressionWormMutationExon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable SinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β2B Tubulin
Plasmid#170553PurposeEncodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.DepositorTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Wild-type CoV-2 ORF8
Plasmid#159933PurposeProtein overexpression in E.coliDepositorInsertCoV-2 ORF8 18-121 (ORF8 CoV-2)
Tags6x Histidine, TEV cleavableExpressionBacterialMutationNonePromoterT7Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
DYR1A_HUMAN_D0
Plasmid#79690PurposeThis plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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SRPK2
Plasmid#39047PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSRPK2 (SRPK2 Human)
TagsHis TevExpressionBacterialMutationInternal segment 268-518 deleted; codon-optimizedAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
ATAD2
Plasmid#38916PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
FH
Plasmid#38978PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
PYCR1
Plasmid#38842PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
MAT1A
Plasmid#38852PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
USP08 (3N3K)
Plasmid#28115PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUSP08 (USP8 Human)
TagsHis tag with TEV siteExpressionBacterialMutationCatalytic domain only.Available SinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
human TLNRD1-F250D pET151
Plasmid#159385PurposeExpresses human TLNRD1 F250D mutant in bacterial cellsDepositorInsertTalin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)
TagsHis-tag, TEV cleavage siteExpressionBacterialMutationChanged Phenylalanine 250 to Aspartic Acid (F250D)PromoterT7Available SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG610
Plasmid#40195DepositorInsertMex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
TagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa 1-355; exon 2 recoded to be RNAi-resi…Promoter4.4 kb mex-5 promoter fragmentAvailable SinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEG585
Plasmid#40192DepositorInsertMex-5 (S404A) (RNAi resistant) (mex-5 Nematode)
TagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationS404A; exon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable SinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTH
Plasmid#200158PurposeFor bacterial expression of the APOBEC1-YTH fusion protein for protein purificationDepositorInsertAPOBEC1-YTH
Tags6xHis, HA, and Maltose Binding ProteinExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-PelB-6H-Ag43-160N-sfGFP
Plasmid#172886PurposeDisplay sfGFP protein on E. coli cell surfaceDepositorInsertsTagsHHHHHH and TEV recognition site (ENLYFQG)ExpressionBacterialMutationDeleted 1-160 (N terminal)PromoterT7 PromoterAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
zCD2
Plasmid#122031PurposeExpresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.DepositorInsertZebrafish HDAC6 Catalytic Domain 2
TagsHis6-MBP tag with cleavable TEV siteExpressionBacterialPromoterT7Available SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin K63C
Plasmid#86589PurposeBacterial expression of Ubiquitin K63C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
TagsHis6-MBPExpressionBacterialMutationTEV site; K63CPromoterTacAvailable SinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits