Addgene

We narrowed to 2,313 results for: PRK

Showing: 2281 - 2313 of 2313 results

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pDONR223-MAP2K5

Plasmid

#23805

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP2K5 (MAP2K5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag GRK6

Plasmid

#20496

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

GRK6 (GRK6 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-CIB1

Plasmid

#23364

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CIB1 (CIB1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_MAP2K4_WT

Plasmid

#82142

Purpose

Gateway Donor vector containing MAP2K4 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K4 (MAP2K4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

P27R; p.8delG

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_AKT3_WT

Plasmid

#82093

Purpose

Gateway Donor vector containing AKT3 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT3 (AKT3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_MAPK6_p.D387N

Plasmid

#81712

Purpose

Gateway Donor vector containing MAPK6, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK6 (MAPK6 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

L290V; D387N; del amino acids 1-8

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-MAPK8

Plasmid

#23420

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK8 (MAPK8 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_AKT1_p.Q79K

Plasmid

#81479

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Q79K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-MAPK10

Plasmid

#23649

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK10 (MAPK10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP2K6

Plasmid

#23783

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP2K6 (MAP2K6 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_MAP2K1_p.E203K

Plasmid

#81729

Purpose

Gateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

L197P; E203K; I310N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-MAP2K4-G294E

Plasmid

#116430

Purpose

Lentiviral expression of MAP2K4 G294E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K4 (MAP2K4 Human)

Use

Lentiviral

Tags

Expression

Mutation

G294E

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_AKT1_p.D323Y

Plasmid

#82867

Purpose

Gateway Donor vector containing AKT1, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D323Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-GRK7

Plasmid

#23402

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GRK7 (GRK7 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_AKT1_p.D44N

Plasmid

#81456

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NM_005163.2 (AKT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D44N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-MAPK4

Plasmid

#23441

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK4 (MAPK4 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-GRK6

Plasmid

#23829

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GRK6 (GRK6 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCMV5-FLAG-MKK6-K82M

Plasmid

#21583

Depositor

John Kyriakis

Article

Yuasa et al J Biol Chem. 1998 Aug 28. 273(35):22681-

Insert

MKK6 (MAP2K6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

lysine 82 to methionine

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223_MAP2K4_p.S184L

Plasmid

#81681

Purpose

Gateway Donor vector containing MAP2K4, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K4 (MAP2K4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S184L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag MAP2K6

Plasmid

#20515

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP2K6 (MAP2K6 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pInducer-myc-ERK1-R84S/S170D/D338N

Plasmid

#213595

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D/D338N cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, S170D, D338N

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pInducer-myc-ERK1-R84S/S170D/Y280A

Plasmid

#213596

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D/Y280A cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, S170D, Y280A

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Pan-GRK [N145/20R]

Plasmid

#206536

Purpose

Mammalian Expression Plasmid of anti-Pan-GRK (Mouse). Derived from hybridoma N145/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-GRK (Mus musculus) recombinant Mouse monoclonal antibody (Grk5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry

Plasmid

#181855

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Histidine 33 in DnaJB1 changed to glutamine.

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry

Plasmid

#181854

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Histidine 33 in DnaJB1 changed to glutamine.

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-AKT3-M339I

Plasmid

#116105

Purpose

Lentiviral expression of AKT3 M339I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT3 (AKT3 Human)

Use

Lentiviral

Tags

Expression

Mutation

M339I

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-AKT3-S370P

Plasmid

#116106

Purpose

Lentiviral expression of AKT3 S370P

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT3 (AKT3 Human)

Use

Lentiviral

Tags

Expression

Mutation

S370P

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_AKT1_p.V201I

Plasmid

#81453

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

V201I; p.117delE

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_AKT1_p.R370C

Plasmid

#81416

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R370C; p.114delE

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_AKT3_p.E17K

Plasmid

#82172

Purpose

Gateway Donor vector containing AKT3 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT3 (AKT3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E17K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-AKT1

Plasmid

#23752

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT1 (AKT1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag AKT3

Plasmid

#20423

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AKT3 (AKT3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-AKT3

Plasmid

#23539

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT3 (AKT3 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

S2R

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only