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pJL1-neoR

Plasmid

#117048

Purpose

In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

neoR

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_HOMEZ_1

Plasmid

#86352

Purpose

Encodes gRNA for 3' target of human HOMEZ

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HOMEZ (HOMEZ Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF652_2

Plasmid

#86317

Purpose

Encodes gRNA for 3' target of human ZNF652

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF652 (ZNF652 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_HOMEZ

Plasmid

#86289

Purpose

Donor vector for 3' FLAG tag of human HOMEZ

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

HOMEZ homology arms (HOMEZ Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HOMEZ_2

Plasmid

#86353

Purpose

Encodes gRNA for 3' target of human HOMEZ

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HOMEZ (HOMEZ Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_TFE3_1

Plasmid

#86325

Purpose

Encodes gRNA for 3' target of human TFE3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against TFE3 (TFE3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HBP1_1

Plasmid

#86320

Purpose

Encodes gRNA for 3' target of human HBP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HBP1 (HBP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_ZNF652_1

Plasmid

#86316

Purpose

Encodes gRNA for 3' target of human ZNF652

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF652 (ZNF652 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_HBP1

Plasmid

#86272

Purpose

Donor vector for 3' FLAG tag of human HBP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

HBP1 homology arms (HBP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHL391

Plasmid

#62821

Purpose

Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

gfp
mCherry

Use

Synthetic Biology

Tags

hfp leader, ompC leader, and st7 RBS

Expression

Bacterial

Mutation

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL518

Plasmid

#62823

Purpose

Lim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

mCherry
gfp

Use

Synthetic Biology

Tags

m9::ompC leader and micC leader

Expression

Bacterial

Mutation

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL2021

Plasmid

#62833

Purpose

Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

T710 hfq leader
gfp

Use

Synthetic Biology

Tags

m9::sodB leader

Expression

Bacterial

Mutation

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL2026

Plasmid

#62834

Purpose

Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

T710 hfq leader
gfp

Use

Synthetic Biology

Tags

m9::glmS leader

Expression

Bacterial

Mutation

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL2027

Plasmid

#62835

Purpose

Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

T710 hfq leader
gfp

Use

Synthetic Biology

Tags

glmS leader

Expression

Bacterial

Mutation

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL2053

Plasmid

#62836

Purpose

Lim Lab plasmid: AmpR + PLlacO-1::T710::hfq::T1 termintor + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

T710 hfq leader
gfp

Use

Synthetic Biology

Tags

m9::ompC leader

Expression

Bacterial

Mutation

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL2073

Plasmid

#62837

Purpose

Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

glmZ leader
T710 hfq leader
gfp

Use

Synthetic Biology

Tags

m9::glmS

Expression

Bacterial

Mutation

Promoter

pConShortNoHind, pLlacO-1, and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL2075

Plasmid

#62838

Purpose

Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

glmZ leader
T710 hfq leader
gfp

Use

Synthetic Biology

Tags

glmS

Expression

Bacterial

Mutation

Promoter

pConShortNoHind, pLlacO-1, and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL2085

Plasmid

#62840

Purpose

Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

m8::glmZ leader
T710 hfq leader
gfp

Use

Synthetic Biology

Tags

glmS

Expression

Bacterial

Mutation

Promoter

pConShortNoHind, pLlacO-1, and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL2117

Plasmid

#62842

Purpose

Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

M8::ryhB leader
gfp

Use

Synthetic Biology

Tags

m9::sodB leader

Expression

Bacterial

Mutation

Promoter

pConShortNoHind and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL2084

Plasmid

#62839

Purpose

Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

m8::glmZ leader
T710 hfq leader
gfp

Use

Synthetic Biology

Tags

m9::glmS

Expression

Bacterial

Mutation

Promoter

pConShortNoHind, pLlacO-1, and pLtetO-1

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only