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SuExp His-Myc-mPLD3

Plasmid

#173853

Purpose

Protein expression plasmid for recombinant His-Myc tag mouse PLD3

Depositor

David Nemazee

Article

Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

Insert

PLD3 (Pld3 Mouse)

Use

Tags

6xHis, Myc, Factor X cleavable

Expression

Mammalian

Mutation

intracellular and transmembrane domain truncated,…

Promoter

CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ eiF4A1 myc HIS

Plasmid

#71657

Purpose

mammalian expression of eiF4A1

Depositor

Gideon Dreyfuss

Insert

eiF4A1 (EIF4A1 Human)

Use

Tags

HIS and myc

Expression

Mammalian

Mutation

aa403 from Ala to Arg

Promoter

CMV

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pDD287 (mKate2^SEC^3xMyc

Plasmid

#70685

Purpose

mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mKate2

Expression

Worm

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRalpha-V242K

Plasmid

#136436

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRalpha (PDGFRA Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

V242K (disrupts PDGF binding, but still binds HCM…

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab5a-c-Myc

Plasmid

#79801

Purpose

Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab5a (RAB5A Human)

Use

Tags

TagBFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pBABE-hygro-2xMyc-Rac1-WT

Plasmid

#128580

Purpose

For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLX208_CMV::mCherry-myc-VAMP2

Plasmid

#199716

Purpose

Expresses VAMP2, fused to mCherry, in mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-myc-VAMP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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N-Myc-53BP1 WT pLPC-Puro

Plasmid

#19836

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Oct 19. ():.

Insert

p53 Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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#2.4 pre-G-SynapShot-Myc

Plasmid

#214722

Purpose

Expresses green #2.4 pre-SynapShot and Myc tag

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#2.4 pre-G-SynapShot

Use

AAV

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His beta4

Plasmid

#16039

Depositor

Filippo Giancotti

Article

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

beta4 (ITGB4 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pTRIPZ Myc2-mouse TTP WT

Plasmid

#107000

Purpose

Expresses epitope-tagged wild-type murine TTP

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

tristetraprolin (Zfp36 Mouse)

Use

Tags

Double Myc peptide epitope tag

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-GFP-MYC

Plasmid

#185375

Purpose

For mammalian expression of human GFP-MYC

Depositor

Ayaz Najafov

Insert

MYC (MYC Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2/myc-His

Plasmid

#127486

Purpose

Expresses myc-tagged NDUFS2 in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Myc-YAP-5SA

Plasmid

#33093

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Promoter

CMV/MSV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-Hygromycin-sfGFP-MAP

Plasmid

#44100

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Use

Tags

sfGFP-MAP

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc-tagged PSK

Plasmid

#197149

Purpose

Expression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cells

Depositor

Jonathan Morris

Article

Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

Insert

TAOK2 (TAOK2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Wild Type

Promoter

SV40

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HLA-cMyc-EcopT1R1

Plasmid

#113962

Purpose

mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R1 (TAS1R1 Human)

Use

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

truncate N-terminal 24 residues

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_535-1102 USP7

Plasmid

#131246

Purpose

Mammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Expresses USP7 amino acids 535-1102. This truncat…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-CD63-FKBP-Myc

Plasmid

#177910

Purpose

human CD63-FKBP-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD63 (CD63 Human)

Use

Tags

FKBP and Myc tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDD286 (TagRFP^SEC^3xMyc)

Plasmid

#70684

Purpose

TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.

Depositor

Bob Goldstein

Insert

TagRFP-T-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized TagRFP-T

Expression

Worm

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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