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LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode

Plasmid

#170226

Purpose

Barcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Publication

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

IKBKB (IKBKB Human)

Use

Lentiviral

Tags

FLAG

Mutation

S177E, S181E

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-YdLight1

Plasmid

#125705

Purpose

Expresses the yellow genetically encoded dopamine sensor YdLight1 in mammalian cells.

Depositor

Lin Tian

Publication

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YdLight1

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ145-ZmUbi-tRNA

Plasmid

#158403

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST P38KTRDronpa

Plasmid

#205763

Purpose

Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Publication

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

P38 KTR Dronpa

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST ERKKTRrsFastLime

Plasmid

#205765

Purpose

Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Publication

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

ERK KTR rsFastLime

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ5135: pCAG-BFP-GuideArray

Plasmid

#210606

Purpose

Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

Depositor

Stanley Qi

Publication

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCAG-BFP-GuideArray

Use

CRISPR

Promoter

CAG

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA

Plasmid

#194723

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1

Depositor

Rudolf Jaenisch

Publication

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AsCpf1 (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro

Plasmid

#192684

Purpose

Lentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNAs #1,2,3 (IL1RN Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

Plasmid

#192682

Purpose

Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-scFv-VP64_Blast

Plasmid

#192655

Purpose

3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

scFv-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_NES_STf

Plasmid

#182001

Purpose

CMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines

Depositor

Kai Johnsson

Publication

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

NES-STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1_STf

Plasmid

#182006

Purpose

CMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines

Depositor

Kai Johnsson

Publication

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LAMP1_STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3806

Plasmid

#185747

Purpose

Sub-array spacer 2/4

Depositor

Rodrigo Ledesma-Amaro

Publication

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 2/4

Use

CRISPR

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:Lucia:bgH

Plasmid

#135293

Purpose

Transcriptional unit for constitutive expression of Lucia luciferase in mammalian cells

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CMV:Lucia:bGH

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pECO300

Plasmid

#169766

Purpose

Express SpCas9 and Kanamycin resistance gene

Depositor

Sang-Gyu Kim

Publication

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Plant binary vector

Expression

Plant

Promoter

35S, AtU6

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1/Pur

Plasmid

#171682

Purpose

constitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin)

Depositor

Randy Poon

Publication

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

OsTIR1-MYC

Tags

MYC

Expression

Mammalian

Promoter

EF-1a promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1-tTA/Neo

Plasmid

#171684

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)

Depositor

Randy Poon

Publication

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

Neomycin

Expression

Mammalian

Promoter

RPBSA promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pVP16 AD

Plasmid

#165822

Purpose

VP16 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

VP16 AD

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCSN074

Plasmid

#166731

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Publication

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMiniWhiteA2

Plasmid

#165873

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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