We narrowed to 6,084 results for: nls

1. pLVX-TetOne-zeo_3xHA-TurboID-NLS

   Plasmid

   #200022

   Purpose

   For lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoter

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   3xHA-TurboID-NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3Gs

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIG-V5-Snail2-IRES-nls-GFP

   Plasmid

   #44282

   Depositor

   Martin Cheung

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

   Insert

   V5-Snail2 (SNAI2 Chicken)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE-enhancer and chicken beta-actin promoter

   Available since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-NLS-copGFP-EF1-BlastiS

   Plasmid

   #132772

   Purpose

   Spills into the cytoplasm during nuclear envelope rupture.

   Depositor

   Jan Lammerding

   Article

   Denais et al Science. 2016 Apr 15;352(6283):353-8. do

   Insert

   NLS-copGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

   Plasmid

   #127362

   Purpose

   gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. phage UBC NLS-HA-2XMCP-tagRFPt

   Plasmid

   #64541

   Purpose

   Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP.

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   MCP
   

   Use

   Lentiviral

   Tags

   HA, NLS, and tagRFP-T

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. phage ubc nls ha pcp gfp

   Plasmid

   #64539

   Purpose

   Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   PCP
   

   Use

   Lentiviral

   Tags

   EGFP, FactorXa site, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-NLS-CBGreen-FIRE-puro

   Plasmid

   #139195

   Purpose

   Luminescent reporter of ERK activity

   Depositor

   Matthew Lazzara

   Article

   Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

   Insert

   CBG99
   

   Use

   Luciferase and Retroviral

   Tags

   Fra1 PEST domain fragment (FIRE) and nuclear loca…

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3XFLAG-VP64-SadCas9-NLS-VP64

   Plasmid

   #135338

   Purpose

   3xFLAG 2xVP64 SadCas9 for single guide cloning

   Depositor

   Ronald Cohn

   Article

   Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

   Insert

   SadCas9
   

   Use

   Tags

   3xFLAG-VP64-SV40 NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX_TRC311/NLS-Cas13d-P2A-Blast

   Plasmid

   #212196

   Purpose

   CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40

   Depositor

   Stacey Edwards

   Article

   Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:

   Insert

   CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
   

   Use

   CRISPR and Lentiviral

   Tags

   HA, NLS, and blasticidin S deaminase

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAY5 (mAID-EGFP-NLS piggyBac)

    Plasmid

    #140532

    Purpose

    mAID-EGFP-NLS piggyBac

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    mAID-EGFP-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CROPseq-multi-EBFP2-NLS (pRW1447)

    Plasmid

    #216219

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PET-B-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207377

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207374

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AsCas12a-6xNLS-E174R/S542R (pRG232)

    Plasmid

    #149723

    Purpose

    Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

    Depositor

    Junwei Shi

    Article

    Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAVf-EnhCB-lacZnls miR-122 3x

    Plasmid

    #35644

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    3 miR-122 target sites
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CB

    Available since

    April 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG

    Plasmid

    #225245

    Purpose

    Mammalian expression of iPASnFR-H in the nucleus

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

    Plasmid

    #86827

    Purpose

    Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    FLAG-NLS-MS2-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pUBC-SV40NLS-Pfv-Sapphire-PURO

    Plasmid

    #203654

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. NLS-Laconic/pCMV-myc-nuc

    Plasmid

    #46307

    Purpose

    a nuclear-targeted, genetically-encoded sensor for lactate

    Depositor

    Luis Felipe Barros

    Article

    Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

    Insert

    NLS-Laconic
    

    Use

    Lactate biosensor

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid

    #181743

    Purpose

    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    bacterial codon optimized SpRY
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS(SV40)-6xHis and MKIEE MBP tag

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    T7

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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