We narrowed to 6,447 results for: nls

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pAAV-CAG-NLS-QPAS1-VP16

Plasmid

#186187

Purpose

AAV vector packaging QPAS1-VP16, a component of optogenetic system for NIR light-controllable transcriptional activation

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

Insert

NLS-QPAS1-VP16

Use

AAV

Promoter

CAG

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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CDS_Cas9-NLS

Plasmid

#136121

Purpose

Puchta Arabidopsis codon-optimised Cas9 for CRISPR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_AtcoCas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CTAG_NLS-SV40

Plasmid

#136108

Purpose

CTAG Nuclear localiation signal SV40 for fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_NLS-SV40

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-synapsin-NLS-mTurquoise2-optoNES-WPRE

Plasmid

#159941

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-YPET-optoNES-WPRE

Plasmid

#159942

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mScarlet-optoNES-WPRE

Plasmid

#159943

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mScarlet-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-Dronpa-optoNES-WPRE

Plasmid

#159944

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-Dronpa-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pED55 pRS306 pRPS2-SteND-NLS-mCherry

Plasmid

#68277

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7ND NLS mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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MMW948: CAG-DmrC-NLS-3xFLAG-VPR

Plasmid

#104565

Purpose

Mammalian expression vector for DmrC fused to VPR activator

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

DmrC-VPR

Tags

3x FLAG and NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-NS1

Plasmid

#175276

Purpose

AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)

Use

AAV

Tags

NLS-dTomato (P2A cleavage)

Promoter

Synapsin

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-PKIalpha-IRES-nls-mRuby2

Plasmid

#63059

Purpose

Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

PKIalpha (Pkia Mouse, Synthetic)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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HeFSpCas9

Plasmid

#92355

Purpose

Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

Cbh

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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eA3A-BE3 (pRZ206)

Plasmid

#131315

Purpose

CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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L_NLS 67kDa

Plasmid

#201361

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-6PrA

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS 41kDa

Plasmid

#201341

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO_Ollas.S.V5_mCherry-NLS

Plasmid

#178211

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

Nuclear Localization Signal and Ollas.S.V5

Promoter

eF1a

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

Plasmid

#179586

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NeonGreen

Promoter

TRE

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.HSV.S_mCherry-NLS

Plasmid

#178224

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HSV.S and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.C.VSVg_mCherry-NLS

Plasmid

#178214

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.C.VSVg and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD_NLS

Plasmid

#124704

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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