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pET28_Caspase-4_W267N

Plasmid

#214314

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

W267N

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_R269D

Plasmid

#214313

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

R269D

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDL28_Cas9-His Δcys E532C/E1207C

Plasmid

#206289

Purpose

Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S, E1207C

Promoter

T7

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDL27_Cas9-His Δcys E532C/E945C

Plasmid

#206288

Purpose

Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S, E945C

Promoter

T7

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDL26_Cas9-His Δcys M1C/E532C

Plasmid

#206287

Purpose

Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

M1C, C80S, E532C, C574S

Promoter

T7

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-TEV-AtPCO5(wt)

Plasmid

#191803

Purpose

Full length wt AtPCO5 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO5

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Melissa Jurica, Tara Davis

Article

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Use

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-Kindlin

Plasmid

#186136

Purpose

For expression of recombinant Snap-labeled kindlin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

FERMT2 (FERMT2 Human)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJK130

Plasmid

#71129

Purpose

Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide synthetase

Use

Tags

His6

Expression

Bacterial

Mutation

changed valine-319 to phenylalanine

Promoter

T7

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET-GST-TNRC6A-(935-984)-NES-mut

Plasmid

#42051

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

GST and His

Expression

Bacterial

Mutation

contain aa935-984, F951A, F955A, I958A, F960A

Promoter

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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p40deltaPX (w/out aa1-135)

Plasmid

#19019

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox (NCF4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

aa136-148 (PX domain, truncated)

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pCpSRb-4

Plasmid

#13726

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRP102 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Circularly permuted SRβD210K183 with Precission p…

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCpSRb-5

Plasmid

#13727

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRP102 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

SRβ circularly permuted D210E188 improved solubil…

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pDrSR beta deltaN

Plasmid

#13730

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit

Use

Tags

6xHis

Expression

Bacterial

Mutation

expresses residues 60-266 of Danio rerio SRβ with…

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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PET-28a-YTH domain of YTHDF2

Plasmid

#228776

Purpose

Protein expression plasmid for YTH domain of YTHDF2 in E.Coli.

Depositor

Huiqing Zhou

Article

Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

Insert

YTH domain of YTHDF2 (YTHDF2 Human)

Use

Tags

6*His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_NoMTS_pET28

Plasmid

#34705

Depositor

David Chan

Article

Ngo et al Nat Struct Mol Biol. 2011 Oct 30;18(11):

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Encodes residues 43–246, corresponding to full-le…

Promoter

T7

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop A

Plasmid

#171779

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop A

Use

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Val2…

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-AviTag-DogCatcher-MBP

Plasmid

#171928

Purpose

Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

AviTag-DogCatcher-MBP

Use

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

DogCatcher added to N-terminus of MBP via a GSGES…

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1363

Plasmid

#166861

Purpose

Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp8 (ORF1ab Synthetic)

Use

Tags

His-GB1

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1362

Plasmid

#166860

Purpose

Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp7 (ORF1ab Synthetic)

Use

Tags

His-GB1

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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