We narrowed to 5,516 results for: pcas

1. pCAG-Gal4DBD-GBP2

   Plasmid

   #49439

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP2-Gal4 DNA binding domain fusion protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAX APP delta CT

   Plasmid

   #30143

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of C-terminal intracellular domain (afte…

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pX330-Flag-deSpCas9

   Plasmid

   #92114

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-CD4-myc

   Plasmid

   #58537

   Purpose

   Expresses human CD4 with a C-terminal myc tag in mammalian cells

   Depositor

   Jacob Yount

   Article

   Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

   Insert

   CD4 (CD4 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

5. eSpCas9(1.1)_No_FLAG

   Plasmid

   #79877

   Purpose

   FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG_Xph15-eGFP-CCR5TC

   Plasmid

   #135528

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCasper_Pdf-LexA::VP16

   Plasmid

   #33259

   Depositor

   Michael Rosbash

   Article

   Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;10

   Insert

   LexA::VP16-SV40 3'UTR (lexA E. coli)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Pdf

   Available since

   Dec. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCAG vGlut1 GCaMP5G

   Plasmid

   #105008

   Purpose

   Presynaptic calcium sensor

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   SLC17A7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Ace2_D92N_E199V-mNeon

   Plasmid

   #129256

   Purpose

   Express Ace2_D92N_E199V-mNeon in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

   Insert

   Ace2_D92N_E199V-mNeon
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D92N, E199V

   Promoter

   CAG

   Available since

   Oct. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAX APP C60

    Plasmid

    #30152

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein C60
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fragment

    Promoter

    Available since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

11. hGli3 NTOTAL in AINGpCAGGS

    Plasmid

    #121973

    Purpose

    medium Gli3 transcriptional repressor

    Depositor

    James Briscoe

    Article

    Ulloa et al Curr Biol. 2007 Mar 20;17(6):545-50. doi

    Insert

    hGli3 NTOTAL (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc and Linker A IRES NLS GFP pCAGGS

    Expression

    Mammalian

    Mutation

    C terminus deletion from zinc finger domain

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-nDsRedFL-intron

    Plasmid

    #177804

    Purpose

    An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    intron
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-GFP-G3BP1(2)

    Plasmid

    #136059

    Purpose

    G3BP1 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTATTACACACTGCTGAACC)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-GST FNIP2

    Plasmid

    #164982

    Purpose

    FNIP2 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FNIP2 (FNIP2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAS9-mCherry-TUBB

    Plasmid

    #66942

    Purpose

    CRISPaint target selector TUBB

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA TUBB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFREE2-SpCas9-sgRNA

    Plasmid

    #179582

    Purpose

    Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-HA-NLGN3 R451C

    Plasmid

    #59319

    Purpose

    Expression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutation

    Depositor

    Peter Scheiffele

    Article

    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

    Insert

    NLGN3 R451C (NLGN3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Arginine 451 to Cysteine (R451C mutation)

    Promoter

    CAG

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGIG-6xHIS.Mm.RPE65-eGFP

    Plasmid

    #41019

    Depositor

    John Nickerson

    Insert

    RPE65 (Rpe65 Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS 3xFLAG Mbnl2

    Plasmid

    #217032

    Purpose

    Overexpression of Mbnl2 in mammalian cells

    Depositor

    Chaolin Zhang

    Article

    Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

    Insert

    Mbnl2 (Mbnl2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-mDrc7-FLAG

    Plasmid

    #176470

    Purpose

    Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    dynein regulatory complex subunit 7 (Drc7 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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