We narrowed to 5,817 results for: pcas

1. pCAG/ATP11C(E184Q)-HA

   Plasmid

   #209251

   Purpose

   Mammalian expression of ATP11C mutant E184Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP11C (ATP11C Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E184Q

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG/ATP8A1(E191Q)-HA

   Plasmid

   #209240

   Purpose

   Mammalian expression of ATP8A1 mutant E191Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP8A1 (ATP8A1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E191Q

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

   Plasmid

   #86612

   Purpose

   Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via nhej using ouabain

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-OSF-VPS15

   Plasmid

   #99326

   Purpose

   Component for mammalian expression of recombinant PI3KC3-C1 complex

   Depositor

   James Hurley

   Article

   Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

   Insert

   VPS15 (PIK3R4 Human)
   

   Tags

   Strep-Strep-Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

5. U6-hGRIN2B-CAG-ps-SpCas9

   Plasmid

   #102851

   Purpose

   A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR

   Tags

   3X Flag and NLS

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-spCas9-pNeo

   Plasmid

   #214120

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   spCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-mCherry2-mito

   Plasmid

   #164164

   Purpose

   Expresses mCherry2-fused with a mitochondria-targeting signal of the ActA gene of Listeria monocytogenes under the control of CAG promoter

   Depositor

   Isei Tanida

   Article

   Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

   Insert

   mCherry2-mtActA
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ER50-SpCas9-ER50

   Plasmid

   #85448

   Purpose

   Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   ER50-SpCas9-ER50
   

   Use

   AAV

   Tags

   destabilized domain of estrogen receptor ligand b…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpCas9-CBE6a-IVT

   Plasmid

   #215833

   Purpose

   Template for in vitro transcription of CBE6a (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Myc-Oct3/4-IP

    Plasmid

    #13460

    Depositor

    Shinya Yamanaka

    Article

    Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

    Insert

    Oct3/4 (Pou5f1 Mouse)
    

    Tags

    Myc (6x)

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-OSF-BECN1

    Plasmid

    #99328

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    Beclin 1 (BECN1 Human)
    

    Tags

    Strep-Strep-Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pCALNL-Rheb(S16H)

    Plasmid

    #194204

    Purpose

    Cre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.

    Depositor

    Angelique Bordey

    Article

    Lin et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    Ras Homolog Enriched Protein in Brain (S16H) (RHEB Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Mutation

    S16H

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

    Plasmid

    #110302

    Purpose

    Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Expression

    Mammalian

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-HA-Nrxn1α-LNS3

    Plasmid

    #128107

    Purpose

    Express HA tagged Nrxn1α-LNS3

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS3 (Nrxn1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-HA-Nrxn1α-LNS1

    Plasmid

    #128105

    Purpose

    Express HA tagged Nrxn1α-LNS1

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS1 (Nrxn1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNS20-SpCas9-SNAP

    Plasmid

    #113717

    Purpose

    Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA24N_6xHis-TEV-rpsA

    Plasmid

    #83585

    Purpose

    Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

    Depositor

    Nils Walter

    Article

    Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

    Insert

    rpsA (rpsA Escherichia coli)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    p-T5 Lac

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG/ATP11A(E186Q)-HA

    Plasmid

    #209249

    Purpose

    Mammalian expression of ATP11A mutant E186Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP11A (ATP11A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E186Q

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-cofilin-S3D

    Plasmid

    #78297

    Purpose

    Mammalian expression of mouse cofilin S3D fused to EGFP

    Depositor

    Michael Frotscher

    Article

    Chai et al Development. 2016 Mar 15;143(6):1029-40.

    Insert

    cofilin 1 (Cfl1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S3D

    Promoter

    CAG

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-OSF-VPS34

    Plasmid

    #99327

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    VPS34 (PIK3C3 Human)
    

    Tags

    Strep-Strep-Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only