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pENTR1A hu_ncOGT WT

Plasmid

#154281

Purpose

Entry vector encoding non-tagged O-GlcNAc Transferase

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Mutation

WT OGT

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE

Plasmid

#174610

Purpose

PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

human CD19, antigen minigene

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-mcherry-2a-CDK2

Plasmid

#121131

Purpose

neutrophil specific expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

dre-WT CDK2 (cdk2 Zebrafish)

Tags

mcherry-2a

Expression

Bacterial

Promoter

neutrophil specific lyzC

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP2s

Plasmid

#101276

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Expression

Mammalian

Promoter

hSyn (human synapsin)

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Designed VEGF-binding scFv G6des13

Plasmid

#110213

Purpose

Yeast Surface Display of G6des13

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6des13

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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Cytosolic Fyn FRET Biosensor

Plasmid

#124654

Purpose

FRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet

Depositor

Yingxiao Wang

Article

Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

Insert

Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet

Expression

Mammalian

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBB75-ncRNA

Plasmid

#194087

Purpose

Expresses Ec86 ncRNA in Escherichia coli

Depositor

Tingting Zou

Article

Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

Insert

Ec86 ncRNA

Promoter

T7

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pSW646 - pML1>>

Plasmid

#115995

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Tags

HDEL and SP(ER)

Expression

Plant

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Stop-EGFP (MSE)

Plasmid

#154469

Purpose

MSCV-Stop-EGFP-pgk-Neo (retroviral construct containing the RFP-based translational stop cassette)

Depositor

Cornelius Miething

Article

Shoumariyeh et al Oncogene. 2020 Feb;39(9):1904-1913. doi:

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-mEGFP

Plasmid

#185013

Purpose

Bacterial Expression of 6xHis Sirius-GFP Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

Sirius-GFP

Expression

Bacterial

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B15

Plasmid

#135513

Purpose

Mammalian expression of myc-tagged HLA-B*15:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*15:01 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-2B-Tbx5

Plasmid

#98619

Purpose

Gateway entry vector TBX5

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

TBX5 (Tbx5 Mouse)

Use

Entry vector

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PS

Plasmid

#136358

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-hCRACR2A-isoform-a

Plasmid

#79593

Purpose

Transient expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delCAT

Plasmid

#173942

Purpose

Bacterial expression of PARP1 lacking CAT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delCAT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 661-1014

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-AnillinFL

Plasmid

#187271

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Promoter

U6, CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0-Anillin shRNA

Plasmid

#187270

Purpose

Lentiviral expression of shRNA targeting Anillin

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

shRNA for Anillin (ANLN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-TFEC

Plasmid

#178447

Purpose

doxycycline-inducible expression of Human TFEC in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

TFEC (TFEC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pClneoFLAG human Dendrin

Plasmid

#47934

Purpose

expresses FLAG tagged human Dendrin in mammalian cells

Depositor

Yutaka Hata

Article

Kawata et al J Biochem. 2006 May;139(5):931-9.

Insert

Dendrin-1 (DDN Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains amino acids 2-711 of human Dendrin

Promoter

CMV

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only
pHEN2 F8-2

Plasmid

#186033

Purpose

Express VHH F8-2 with 3 c-myc tag in E. coli periplasm

Depositor

Isabelle Landrieu

Article

Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

Insert

VHH F8-2

Tags

6His and Myc

Expression

Bacterial

Promoter

lac

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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