We narrowed to 16,190 results for: LEA

Showing: 11641 - 11660 of 16190 results

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pDEST14-Cys-SpyCatcher002

Plasmid

#102829

Purpose

Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

Cys-SpyCatcher002

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Cysteine residue in N-terminal region

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCA24N_6xHis-TEV-rpsA

Plasmid

#83585

Purpose

Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

Depositor

Nils Walter

Article

Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

Insert

rpsA (rpsA Escherichia coli)

Tags

His tag

Expression

Bacterial

Promoter

p-T5 Lac

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBG100-TCPTP

Plasmid

#33365

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

TCPTP-45 (PTPN2 Human)

Tags

6xHIS - cleavable

Expression

Bacterial

Promoter

T7lac

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

Plasmid

#69546

Purpose

Tet-On system expressing MyoD-2A-GFP-2xNLS.

Depositor

Charles Gersbach

Inserts

eGFP
Myod1 (Myod1 Mouse)

Use

Lentiviral

Tags

2x nuclear localization sequence and T2A "sk…

Mutation

Removed stop codon to fuse it to the 2A-eGFPnls i…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTC217

Plasmid

#70018

Purpose

Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (Cas9/sgRNA) + Donor + GVR

Use

CRISPR

Expression

Plant

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pET-6His-MBP-MpAgo

Plasmid

#79249

Purpose

This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

Depositor

Jennifer Doudna

Article

Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

Insert

Marinitoga piezophila Argonaute

Use

CRISPR

Tags

6XHis-tag + MBP + PreScission Cleavage Site

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pNCS mRFP

Plasmid

#91765

Purpose

Bacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)

Depositor

Erik Rodriguez, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Insert

monomeric Red Fluorescent Protein1

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-NES

Plasmid

#36206

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

NES

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pGEmCherry2

Plasmid

#118413

Purpose

Red fluorescent reporter for assessing CRISPR gRNA activity

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-GINKO1

Plasmid

#113111

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-YU2gp160

Plasmid

#100927

Purpose

Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (YU2) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-H-Luc_nucl

Plasmid

#113931

Purpose

Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc_nucl

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Plas-CRISPR_reporter

Plasmid

#157658

Purpose

CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

Depositor

Frank Albert

Article

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

dCAS9-VP64, mCherry, KanMX

Use

Insert storage (replicative in e. coli)

Mutation

N28D in mCherry- please see depositor comments be…

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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6x-His_A1aY1_GFP

Plasmid

#158753

Purpose

Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

6xHistidine_A1aY1_GFP

Tags

6x-Histidine tag, GFP, T7 leader sequence, and Th…

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-BFLF1

Plasmid

#162657

Purpose

Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-smURFP-StagRFP-EKAR-EV

Plasmid

#173019

Purpose

Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

smURFP-StagRFP-EKAR-EV

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10

Plasmid

#127129

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K24R- please see depositor comments

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGSHU

Plasmid

#72244

Purpose

Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
hyg
I-SceI

Expression

Bacterial

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMLM3705

Plasmid

#47754

Purpose

Expresses mammalian cell codon-optimized dCas9-VP64

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

codon optimized dCas9-VP64

Use

CRISPR

Tags

3x FLAG, NLS, and VP64

Expression

Mammalian

Mutation

D10A and H840A mutations in Cas9 (catalytically i…

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV2-hNCOA6:V5

Plasmid

#63585

Purpose

A subunit of the Trithorax-related (Trr) methyltransferase complex.

Depositor

Kenneth Irvine

Article

Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

Insert

NCOA6 (NCOA6 Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only