We narrowed to 20,122 results for: Kin

1. pDNR-SpCas9-Cdt1

   Plasmid

   #80426

   Purpose

   Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Cdt1
   

   Use

   CRISPR

   Tags

   Cdt1

   Mutation

   codon optimized for human

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV.5’VIM-eGFP-KI

   Plasmid

   #170547

   Purpose

   Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Inserts

   Left Homology arm for VIM knockin
   Right Homology arm for VIM knockin
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRBV6-5

   Plasmid

   #123425

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV6-5 (TRBV6-5 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-G88P7-rM3Ds-2A-EYFP

   Plasmid

   #213970

   Purpose

   AAV vector with G88P7 promoter in order to induce robust expression of rM3Ds in MSNs

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Insert

   rM3Ds
   

   Use

   AAV

   Promoter

   G88P7

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2

   Plasmid

   #69625

   Purpose

   A FRET sensor for Galphai3 activation, encoded on a single plasmid.

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Inserts

   Gbeta-2A-cpV-Ggamma2
   Galphai3-mTurquoise2
   

   Tags

   ciruclar permutated Venus (cp173V) and mTurquoise…

   Expression

   Mammalian

   Promoter

   CMV and CMV (IRES)

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-SaCas9-U6-sgGabrg2

   Plasmid

   #124857

   Purpose

   Mutagenesis of Gabrg2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg2 (Gabrg2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4-ATR-C-KD

   Plasmid

   #53770

   Purpose

   mammalian expression of flag-tagged ATR C-terminal fragment kinase dead mutant

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

   Insert

   ATR (ATR Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C-terminal domain, aa 1398-2644, and kinase dead …

   Promoter

   CMV

   Available Since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

8. AAV-hSyn-DIO-mCReP

   Plasmid

   #167503

   Purpose

   Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

   Depositor

   Nicole Calakos

   Article

   Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

   Insert

   Ppp1r15b (Ppp1r15b Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR LAT/Zap70 iLID-Drop

   Plasmid

   #171038

   Purpose

   Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-labeled.

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

   Insert

   LAT (LAT Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-HA-Akt1-K179M

    Plasmid

    #73409

    Purpose

    Expresses kinase-inactive mutant of Akt with HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed K179M to make Kinase inactive mutant

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQHA-USP7 CS puroR

    Plasmid

    #46754

    Purpose

    Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C223S--catalytically inactive

    Promoter

    CMV

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cav2.2 pcDNA3

    Plasmid

    #206072

    Purpose

    expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

    Depositor

    Annette Dolphin

    Article

    Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRAV16

    Plasmid

    #123389

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV16 (TRAV16 Human)
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRAV13-1

    Plasmid

    #123386

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV13-1 (TRAV13-1 Human)
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-MED18

    Plasmid

    #15419

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED18 (MED18 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcdna3 flag - ULK2 wt

    Plasmid

    #27637

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse ULK2 (Ulk2 Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Initial M removed from ULK2.

    Available Since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pFUW-tetO-Irf1

    Plasmid

    #139833

    Purpose

    doxycycline-inducible expression of mouse Irf1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Irf1 (Irf1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX_TRC208-SYNJ2BP-V5

    Plasmid

    #92149

    Purpose

    Expresses V5-tagged human SYNJ2BP in mammalian cells.

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    SYNJ2BP-V5 (SYNJ2BP Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CMV

    Available Since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Rab8WT(Flag) in pcDNA3.1neo

    Plasmid

    #46783

    Purpose

    expression of WT Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A (RAB8A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCEP4-CXCR4

    Plasmid

    #98944

    Purpose

    Mammalian expression plasmid for untagged human CXCR4

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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