We narrowed to 23,290 results for: crispr

1. A3C-Cas9n-UGI

   Plasmid

   #119136

   Purpose

   Base editor made from APOBEC3C

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3C-Cas9 nickase-UGI
   

   Use

   CRISPR

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSDMA66

   Plasmid

   #128355

   Purpose

   gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

   Depositor

   James Fraser

   Article

   Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

   Insert

   Noureothricin (NAT)
   

   Use

   Unspecified

   Promoter

   ACT1

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 7xNLS-pMJ915v2-sfGFP

   Plasmid

   #88922

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF711

   Plasmid

   #121662

   Purpose

   U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Inserts

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAIO-AsCpf1

   Plasmid

   #137846

   Purpose

   AsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Insert

   AsCpf1-NLS-FLAG
   

   Use

   CRISPR

   Available Since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH51

   Plasmid

   #128213

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. A3G-Cas9n-UGI

   Plasmid

   #119139

   Purpose

   Base editor made from APOBEC3G

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3G-Cas9 nickase-UGI
   

   Use

   CRISPR

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C-12-BE

   Plasmid

   #135040

   Purpose

   C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   LjCDA1L2_1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 with EF-1alpha intron

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPO636

   Plasmid

   #195299

   Purpose

   pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   TnsABCQ operon of McCAST (Tn7575)
   

   Expression

   Bacterial

   Promoter

   IPTG inducible lac promoter

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p202_LTJ_sgRNACD45.2_R1

    Plasmid

    #82672

    Purpose

    sgRNA targeting murine CD45.2 region 1. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting mouse CD45.2 region 1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRRNA-G0-R0

    Plasmid

    #101803

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    Spy 1049

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. N-8-BE

    Plasmid

    #135023

    Purpose

    C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9

    Depositor

    Tian-Lin Cheng, Zilong Qiu

    Article

    Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

    Insert

    LpCDA1L1_1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 with EF-1alpha intron

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSDMA67

    Plasmid

    #128356

    Purpose

    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

    Insert

    Noureothricin (NAT)
    

    Use

    Unspecified

    Promoter

    ACT1

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDYSCKO

    Plasmid

    #120263

    Purpose

    Empty backbone for cloning any gRNA to be used with the canavanine co-selection system

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    DYSCKO cassette
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    SNR52

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCF713

    Plasmid

    #121665

    Purpose

    U6-sgRNA EF1a-ProCas9-FlaviS6-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Inserts

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCZGY2729

    Plasmid

    #135096

    Purpose

    Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrIV

    Depositor

    Yishi Jin

    Article

    Andrusiak et al Neuron. 2019 Jul 24. pii: S0896-6273(19)

    Insert

    Hygromycin resistance
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    rps-0

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRoDonor

    Plasmid

    #178773

    Purpose

    Expression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

    Depositor

    Chase Beisel

    Article

    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

    Insert

    RoCAST left end, cam, RoCAST right end from Rippkaea orientalis
    

    Expression

    Bacterial

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDA_381

    Plasmid

    #179092

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    IDT-Cas9_v2
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA_157

    Plasmid

    #179090

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    HypaCas9
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRDA_156

    Plasmid

    #179089

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    Cas9-VRER
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge