We narrowed to 23,935 results for: crispr

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3xFLAG-dCas9/p-bacteria

Plasmid

#64325

Purpose

Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

Insert

3xFLAG-dCas9

Use

CRISPR; Tetracycline-inducible

Tags

3xFLAG

Expression

Bacterial

Mutation

D10A, H840A

Promoter

pLtetO-1

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pMB1: All in one SpCas9 expression and sgRNA cloning backbone

Plasmid

#228363

Purpose

mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGH153_MS2-AIDΔ-Hygro

Plasmid

#85416

Purpose

lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AIDΔ and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(TCT1)

Plasmid

#235065

Purpose

Expresses Tad-CBE(TCT1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCT1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-RNF103-CHMP3

Plasmid

#140584

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA RNF103-CHMP3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB6633

Plasmid

#106162

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-hCAS-pA

Plasmid

#48625

Purpose

Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii, Kunihiko Naito

Article

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

FLAG, NLS, and polyA

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP

Plasmid

#69536

Purpose

Episomal plasmid encoding dCas9VP192

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-EGFP

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV_AncBE4max_3xHA

Plasmid

#112097

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCC3)

Plasmid

#235078

Purpose

Expresses Tad-CBE(GCC3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCC3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCG4)

Plasmid

#235080

Purpose

Expresses Tad-CBE(GCG4) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCG4)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pARS3_MUbCAS9_GW

Plasmid

#128646

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

Depositor

G. Eric Schaller

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Tags

HA

Expression

Plant

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pXCas9H840A

Plasmid

#60900

Purpose

Dual Expression Vector for Cas9 H840A nickase and gRNA

Depositor

Bruce Conklin

Article

Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CBh and U6

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pQdCas9-sgempty

Plasmid

#159523

Purpose

Expression of dCas9 driven by lux cassette from Vibrio fischeri

Depositor

Radhakrishnan Mahadevan

Article

Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_ABEmax_3xHA

Plasmid

#112098

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAT15489_dABE8e

Plasmid

#174127

Purpose

plasmid expressing ABE8e with dCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

dABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-UGI-nCas9(H840A)-GSVG

Plasmid

#187411

Purpose

DddA GSVG variant

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA(tyr)

Plasmid

#64250

Purpose

expresses sgRNA(tyr) under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Insert

U6a:sgRNA (tyr)

Use

CRISPR

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAWp28

Plasmid

#73850

Purpose

pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Type

Empty backbone

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

Plasmid

#190794

Purpose

Template vector to amplify pieces for creating multiplex sgRNA-arrays.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA-scaffold only

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23117

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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