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pSEVA221pmCDA1-T7RNAP

Plasmid

#167978

Purpose

Expresses fusion protein pmCDA1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

pmCDA1-T7RNAP (T7p07 Synthetic)

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-eGFP-CCR5TC

Plasmid

#135530

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP2A-EGFP

Plasmid

#168501

Purpose

Expression of full length SRGAP2 with a C-termonal EGFP fusion

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Insert

SRGAP2A (SRGAP2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_sfGPF 150TAG

Plasmid

#154765

Purpose

plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP670nano

Plasmid

#127429

Purpose

Human alpha-tubulin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano-human alpha-tubulin

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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Halo-Cyto

Plasmid

#124314

Purpose

Expresses cytosolic specific HaloTag protein

Depositor

Jin Wang

Article

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Tags

Cytoplasm specific seq

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6011nes

Plasmid

#108652

Purpose

An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-ERK-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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YFP-DAT

Plasmid

#90228

Purpose

Expresses human DAT in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Biol Chem. 2003 Jul 25;278(30):28274-8

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Sico-Red

Plasmid

#84882

Purpose

Cre-inducible shRNA

Depositor

Eun Mi Hwang

Article

Jung et al Exp Neurobiol. 2016 Jun;25(3):120-9. doi

Insert

Sico-Red

Use

AAV

Tags

mCherry

Promoter

EF1a and CMV

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pmcherry-LCa

Plasmid

#53972

Purpose

Expresses the light chain of clathrin

Depositor

Tom Kirchhausen

Article

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

clathrin light chain

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-HisYFP-Sp100

Plasmid

#53141

Purpose

E.coli expression of HisYFP tagged human Sp100 fragment

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

Sp100A fragment (SP100 Human)

Tags

His-YFP

Expression

Bacterial

Mutation

fragment (amino acids 71-480); deletion of L75

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-flag-YTHDF2-C

Plasmid

#52303

Purpose

Flag-tagged YTHDF2 C-terminal domain, from E384 to the end

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

flag

Expression

Mammalian

Mutation

Deleted amino acids 1-383

Promoter

T7

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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SB51

Plasmid

#48184

Purpose

Express full length HA tagged p35sr in mammalian cells

Depositor

Shoumo Bhattacharya

Article

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2014

Availability

Academic Institutions and Nonprofits only
pQTEV-SNX3

Plasmid

#31573

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

sorting nexin 3 (SNX3 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pFBOT563

Plasmid

#40864

Depositor

Harold Gainer

Article

Fields et al PLoS One. 2012;7(2):e32085. Epub 2012 Fe

Insert

Oxytocin promoter

Use

AAV

Expression

Bacterial and Insect

Promoter

mouse oxytocin promoter

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pEntr-Mef2aflbio

Plasmid

#32970

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

Mef2a (MEF2A Human)

Use

Gateway Cloning

Tags

flag-bio

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
LCMV:ECFP(loxP)(FRT)EYFP

Plasmid

#31304

Depositor

Gerhart Ryffel

Article

Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e4

Insert

ECFP(loxP)(FRT)EYFP

Use

Cre/Lox; Flp/frt

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRF+423Dux4

Plasmid

#21625

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

Double homeobox, chr 4 (DUX4 Human)

Use

Luciferase

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
Epsin1-ECFP

Plasmid

#21066

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Epsin 1 (Epn1 Rat)

Tags

ECFP

Expression

Mammalian

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
tetO7_Ef1a_SOX2

Plasmid

#18919

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

SOX2 (SOX2 Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only