173,280 results
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Plasmid#180213PurposeExpressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.ColiDepositorInsertNlaCas5-Cas8-Cas7
Tags6XHis and NLSExpressionBacterialPromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-GFP-NMHC II-A
Plasmid#11347DepositorAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6a
Plasmid#207851PurposeMammalian expression of PE6a prime editorDepositorInsertPE6a
TagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationEc48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…Available SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCasKP-apr
Plasmid#117231PurposeBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella PneumoniaeDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cdc2-HA
Plasmid#1888DepositorAvailable SinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)
Plasmid#163059Purposemito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)DepositorInsertmito-roGFP2-Orp1
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (A7)
Plasmid#174079PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)Available SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXR002: EF1a-dCasRx-2A-EGFP
Plasmid#109050PurposeCatalytically inactive dCasRx with 2A-EGFPDepositorInsertdCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationR239A/H244A/R858A/H863AAvailable SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRECBRR1-Kva1
Plasmid#240703Purposeinverted BRR1 origin of replication plasmid containing Kva1 recombitron with a donor in the ncRNA to target phzM locus in Pseudomonas aeruginosa PAO1 expressed by Pm promoterDepositorInsertKva1 RT,Kva1 ncRNA, PaRecT and PaSSB
ExpressionBacterialMutationInverted BRR1 origin of replicationAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pet-15b-ultraID
Plasmid#172879Purposeexpresses ultraID in E. coliDepositorInsertultraID
TagsmycExpressionBacterialMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only