2,226 results

1. pcDNA3-Lyn-sACt-mCherry

   Plasmid

   #138216

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   Lyn-sACt-mCherry (Adcy10 Rat)
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFSW Doc2alpha WT

   Plasmid

   #128812

   Purpose

   To make virus expressing Doc2alpha WT

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2alpha (Doc2a Rat)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFSW Doc2beta WT

   Plasmid

   #128814

   Purpose

   To make virus expressing Doc2beta WT

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDC184

   Plasmid

   #70055

   Purpose

   Co-expresses Syt1-SNARE37aa-linker complex with p045

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

   Inserts

   His tagged Synaptobrevin 28-89
   Syntaxin 191-256
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Septin 11 isoform III

   Plasmid

   #120319

   Purpose

   Septin 11 isoform III mammalian expression

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 (Sept11 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQZ083

   Plasmid

   #106417

   Purpose

   Express Complexin-1 fragment (1-83) in E. coli

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

   Insert

   complexin-1 fragment 1-83 (Cplx1 Synthetic, Rat)
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Fragment 1-83, Codon optimization

   Promoter

   T7

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCer-Vamp2(qv/fw)

   Plasmid

   #53238

   Purpose

   Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Tags

   4aa-linker and mCerulean

   Expression

   Mammalian

   Mutation

   Mutated Q76 to V; F77 to W.

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pGP-CMV-NES-fRGECO1

   Plasmid

   #125247

   Purpose

   Expresses red fluorescent calcium sensor in mammalian cells

   Depositor

   Katalin Torok

   Article

   Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

   Insert

   fRGECO2
   

   Use

   Tags

   NES signal

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3.1-EPSIN 1

   Plasmid

   #22225

   Depositor

   Pietro De Camilli

   Article

   Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

   Insert

   Epsin 1 (Epn1 Rat)
   

   Use

   Tags

   Xpress

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

10. FKBP-Stx3-mCitrine

    Plasmid

    #92428

    Purpose

    Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx3 (Stx3 Rat)
    

    Use

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLexm-gfp-Pept2

    Plasmid

    #167988

    Purpose

    expresses rat PepT2 with a c terminal cleavable GFP-His tag.

    Depositor

    Simon Newstead

    Article

    Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

    Insert

    PepT2 (Slc15a2 Rat)
    

    Use

    Tags

    cleavable GFP-his8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p3E LAP2b

    Plasmid

    #105978

    Purpose

    3' element for Gateway cloning, lamina-associated polypeptide 2

    Depositor

    Caren Norden

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    LAP2b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-HA-ERK2 L232A

    Plasmid

    #8976

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L232A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pLL3.7 shRNA against CRTC2

    Plasmid

    #72711

    Purpose

    Lentiviral expression of shRNA against CRTC2 (3rd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    CRTC2
    

    Use

    Lentiviral

    Tags

    CMV-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-HA-Nrxn1α-LNS2

    Plasmid

    #128106

    Purpose

    Express HA tagged Nrxn1α-LNS2

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS2 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. eGFP-KCC2

    Plasmid

    #104075

    Purpose

    expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

    Depositor

    Igor Medyna

    Article

    Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

    Insert

    KCC2 (b isoform) (Slc12a5 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EcoR1 site in rat KCC2 was removed by silent muta…

    Promoter

    CMV

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lyn-iTrkA-mCh-CRY2

    Plasmid

    #106169

    Purpose

    mammalian expression of Lyn-tagged intracellular domain of TrkA (aa 450-799) fused to mCherry and CRY2PHR (aa 1-498)

    Depositor

    Bianxiao Cui

    Article

    Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

    Insert

    Lyn iTrkA mCherry CRY2PHR (CRY2 Mustard Weed, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-evoA1-seBEN_empty

    Plasmid

    #174701

    Purpose

    Small-molecule controlled base editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    Lenti-evoA1-seBEN
    

    Use

    Lentiviral

    Tags

    myc tag

    Expression

    Mutation

    Promoter

    EFS/U6

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-evoA1-seBEC

    Plasmid

    #174702

    Purpose

    Small-molecule controlled base editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    Lenti-evoA1-seBEC
    

    Use

    Lentiviral

    Tags

    flag tag

    Expression

    Mutation

    UGI-E11K

    Promoter

    EFS

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNCX2

    Plasmid

    #67148

    Purpose

    Expresses mRNA of NCX2 (Rat brain)

    Depositor

    Michela Ottolia, Kenneth Philipson

    Article

    Li et al J Biol Chem. 1994 Jul 1;269(26):17434-9.

    Insert

    NCX2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge