2,294 results

1. pLenLox_U6 Nlgn3

   Plasmid

   #59359

   Purpose

   Lentiviral expression vector: Neuroligin 3 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

   Depositor

   Peter Scheiffele

   Article

   Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

   Inserts

   Neuroligin 3 shRNA (rat) (Nlgn3 Rat)
   eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CAG and mouse U6

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-RNF34

   Plasmid

   #119938

   Purpose

   RING finger protein 34 expression (HA-tag at the N-terminus)

   Depositor

   Angel de Blas

   Article

   Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

   Insert

   RNF34 (Rnf34 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA-Cx43

   Plasmid

   #60691

   Purpose

   mammalian expression of rat Cx43

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDC184

   Plasmid

   #70055

   Purpose

   Co-expresses Syt1-SNARE37aa-linker complex with p045

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

   Inserts

   His tagged Synaptobrevin 28-89
   Syntaxin 191-256
   

   Tags

   10xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5FRT/TO-p97-EQ-mycStrep

   Plasmid

   #31838

   Depositor

   Hemmo Meyer

   Article

   Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

   Insert

   p97
   

   Tags

   Strep and myc

   Expression

   Bacterial and Mammalian

   Mutation

   E578Q

   Promoter

   CMV

   Available Since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

6. EGFP-Cx43-7

   Plasmid

   #56498

   Purpose

   Localization: Gap Junctions, Excitation: 493, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Cx43
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. mCer-Vamp2(qv/fw)

   Plasmid

   #53238

   Purpose

   Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Tags

   4aa-linker and mCerulean

   Expression

   Mammalian

   Mutation

   Mutated Q76 to V; F77 to W.

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

8. Rat NCX1.3 (pcDNA3.1+)

   Plasmid

   #75191

   Purpose

   Mammalian expression of SLC8A1

   Depositor

   Jonathan Lytton

   Article

   Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

   Insert

   SLC8A1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZac2.1 gfaABC1D-BioID2-BioID2-Kir4.1-HA

   Plasmid

   #176746

   Purpose

   Expresses Kir4.1 fused with BioID2 at the N-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   HA-BioID2-BioID2-Kir4.1 (Kcnj10 Rat, Aquifex aeolicus)
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCWXPGR-pTF-ERK:MEK

    Plasmid

    #114291

    Purpose

    TET-inducible expression of ERK:MEK fusion protein

    Depositor

    Patrick Salmon

    Insert

    rERK2 and hMEK1 proteins (Mapk1 Rat, Human)
    

    Use

    Lentiviral

    Mutation

    rat ERK2 fused to human MEK1 with a linker in bet…

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUp95GW (B7)

    Plasmid

    #74016

    Purpose

    lentiviral expression of shRNA resistant Psd95-EGFP fusion

    Depositor

    Robert Malenka, Weifeng Xu, Oliver Schluter

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    Psd95 (DLG4 Rat)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBactin-Nedd1-mNeonGreen

    Plasmid

    #196864

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mNeonGreen (Nedd1 Rat)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBactin-td-mCherry-Cdk5rap2

    Plasmid

    #196859

    Purpose

    Expression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    td-mCherry-Cdk5rap2 (Cdk5rap2 Rat)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQZ083

    Plasmid

    #106417

    Purpose

    Express Complexin-1 fragment (1-83) in E. coli

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

    Insert

    complexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)
    

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Fragment 1-83, Codon optimization

    Promoter

    T7

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Septin 11 isoform III

    Plasmid

    #120319

    Purpose

    Septin 11 isoform III mammalian expression

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Expression

    Mammalian

    Available Since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Lyn-sACt-mCherry

    Plasmid

    #138216

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    Lyn-sACt-mCherry (Adcy10 Rat)
    

    Tags

    N-terminal targeting sequence from Lyn kinase and…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-CMV-jGCaMP8f F366H

    Plasmid

    #204141

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    CMV

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1-EPSIN 1

    Plasmid

    #22225

    Depositor

    Pietro De Camilli

    Article

    Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

    Insert

    Epsin 1 (Epn1 Rat)
    

    Tags

    Xpress

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

19. EYFP-Lysosomes-20

    Plasmid

    #56595

    Purpose

    Localization: Lysosome Membrane, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. iLID-tdTomato-Omp25

    Plasmid

    #214400

    Purpose

    Expresses fusion of iLID with tdTomato and transmembrane domain of Omp25

    Depositor

    Liting Duan

    Article

    Liu et al Proc Natl Acad Sci U S A. 2024 Apr 2;121

    Insert

    iLID-tdTomato-Omp25 (Synj2bp Rat, Synthetic)
    

    Tags

    iLID-TdTomato-Omp25

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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