Skip to main content
Addgene
Showing: 1161 - 1180 of 8351 results

  1. pJK314

    Plasmid
    #71140
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to valine; additional N-term…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71140-plasmid-map-sequence-id-131039

    Enlarge

  2. pJK174

    Plasmid
    #71835
    Purpose
    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7 and endogenous
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71835-plasmid-map-sequence-id-131052

    Enlarge

  3. pJK279

    Plasmid
    #71135
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to aspartate; additional N-t…
    Promoter
    T7
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71135-plasmid-map-sequence-id-131034

    Enlarge

  4. pJK283

    Plasmid
    #71139
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine; additional N-…
    Promoter
    T7
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71139-plasmid-map-sequence-id-131038

    Enlarge

  5. pJK282

    Plasmid
    #71138
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to aspartate; additional N-t…
    Promoter
    T7
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71138-plasmid-map-sequence-id-131037

    Enlarge

  6. pJK281

    Plasmid
    #71137
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to phenylalanine; additional…
    Promoter
    T7
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71137-plasmid-map-sequence-id-131036

    Enlarge

  7. pJK280

    Plasmid
    #71136
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to phenylalanine; additional…
    Promoter
    T7
    Available since
    Jan. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71136-plasmid-map-sequence-id-131035

    Enlarge

  8. pJK175

    Plasmid
    #71132
    Purpose
    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7
    Available since
    Jan. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71132-plasmid-map-sequence-id-131032

    Enlarge

  13. IgK-IFN-luc

    Plasmid
    #14886
    Purpose
    Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression
    Depositor
    David Baltimore
    Article
    Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.
    Insert
    IgK-IFN (IFNB1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2007
    Availability
    Academic Institutions and Nonprofits only
    14886-plasmid-map-sequence-id-238489

    Enlarge

  14. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190814-plasmid-map-sequence-id-373712

    Enlarge

  15. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Expression
    Mutation
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

    Enlarge

  16. PB-eDIO-EYFP

    Plasmid
    #104388
    Purpose
    PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.
    Depositor
    Volker Busskamp
    Article
    Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10
    Insert
    Enhanced Yellow Fluorescent Protein
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104388-plasmid-map-sequence-id-202374

    Enlarge

Showing: 1161 - 1180 of 8351 results