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2. p167 Dync1i1.D

Plasmid

#26439

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform D (Dync1i1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pLJM1-FH-hnRNPC

Plasmid

#245722

Purpose

Express Flag-HA-hnRNPC in mammalian cells

Depositor

Joshua Mendell

Article

Thomas et al Genes Dev. 2022 May 1;36(9-10):550-565.

Insert

Flag-HA-hnRNPC (HNRNPC Human)

Use

Lentiviral

Tags

Flag-HA

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pDOXOFF (STOP)99xGly

Plasmid

#211349

Purpose

DOX-regulated lentiviral expression of 99xGly-EGFP-3xHA lacking initiation codon

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

(STOP)99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Mutation

Start (ATG) codon replaced with stop (TAG) codon

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5R DD-FAM98B

Plasmid

#211357

Purpose

Transient expression of DD-V5-FAM98B

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

DD-FAM98B (FAM98B Human)

Tags

DD-V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Plasmid

#200792

Purpose

Mammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Δ954-1027

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Plasmid

#200798

Purpose

Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

411-680

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-C-term_F

Plasmid

#146294

Purpose

Mammalian Expression of HsTNRC6A-Cterm

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-Cterm (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delAGOhook_F

Plasmid

#146295

Purpose

Mammalian Expression of HsTNRC6A-delAGOhook

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delAGOhook (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delMI_F

Plasmid

#146296

Purpose

Mammalian Expression of HsTNRC6A-delMI

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMI (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delMII_F

Plasmid

#146297

Purpose

Mammalian Expression of HsTNRC6A-delMII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMII (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-del133-155-V5-SBP-MBP_V

Plasmid

#147763

Purpose

Mammalian Expression of HsSmg5-del133-155

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del133-155 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-del267-809-V5-SBP-MBP_V

Plasmid

#147764

Purpose

Mammalian Expression of HsSmg5-del267-809

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del267-809 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-del810-846-V5-SBP-MBP_V

Plasmid

#147765

Purpose

Mammalian Expression of HsSmg5-del810-846

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del810-846 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-del847-1017-V5-SBP-MBP_V

Plasmid

#147766

Purpose

Mammalian Expression of HsSmg5-del847-1017

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del847-1017 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-del133-155-G120E_T

Plasmid

#147554

Purpose

Mammalian Expression of HsSmg5-del133-155-G120E

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del133-155-G120E (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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UG76-pLYS2-GFP-PKCbeta

Plasmid

#184762

Purpose

Marker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

pLYS2-GFP-PKCbeta (PRKCB Budding Yeast, Human)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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hPCNA-S228I

Plasmid

#190938

Purpose

untagged human PCNA with a hypomorphic disease-associated serine to isoleucine mutation at residue 228

Depositor

Brian Kelch

Article

Duffy et al J Mol Biol. 2016 Mar 27;428(6):1023-1040

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Expression

Bacterial

Mutation

changed serine 228 to isoleucine

Promoter

T7

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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dora[B]_rescue_construct

Plasmid

#190637

Purpose

Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-dora[B]_rescue_construct

Plasmid

#190638

Purpose

Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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dora[A]_rescue_construct

Plasmid

#190639

Purpose

Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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