164,839 results

1. Fuw-dCas9-Tet1CD_IM

   Plasmid

   #84479

   Purpose

   Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Tet1CD_IM
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781)

   Plasmid

   #199777

   Purpose

   enhancer AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs

   Depositor

   Jonathan Ting, Allen Institute for Brain Science

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Has Service

   AAV PHP.eB

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   R297T for reduced recombinase efficiency.

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSPL3-hRPH3A_c.444Gwt

   Plasmid

   #190476

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-GGamma1-GFP2

   Plasmid

   #140989

   Purpose

   Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma1-GFP2 (GNGT1 Human)
   

   Use

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-ABCC1_STOP

   Plasmid

   #221423

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

   Insert

   ABCC1 (ABCC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pet28a_Synthetic_Human_H2A.1

   Plasmid

   #42634

   Depositor

   Joe Landry

   Insert

   Human H2A.1 (H2AC13 Synthetic, Human)
   

   Use

   Tags

   Untagged

   Expression

   Bacterial

   Mutation

   Codon Optimized

   Promoter

   T7

   Available Since

   Feb. 25, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotI

   Plasmid

   #126477

   Purpose

   Expresses Cas9-5xFlag-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   Cas9
   5xFlag
   TdT (DNTT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed codons to reduce repetitive sequences.

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM5057

   Plasmid

   #120085

   Purpose

   Broad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.

   Depositor

   Susan Golden

   Article

   Ma et al Appl Environ Microbiol. 2014 Nov;80(21):

   Insert

   RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR-Flag-IKKalpha

   Plasmid

   #15467

   Depositor

   Hiroyasu Nakano

   Article

   Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

   Insert

   IkB kinase alpha (Chuk Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. Anti-6xHis [N144/14R]

    Plasmid

    #177500

    Purpose

    Mammalian Expression Plasmid of anti-6xHis. Derived from hybridoma N144/14.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-6xHis recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZFN-AAVS1-KKR

    Plasmid

    #159298

    Purpose

    ZFN (right) targeting the AAVS1 locus with obligate heterodimer FokI

    Depositor

    Kosuke Yusa

    Insert

    ZFN targeting the AAVS1 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-OTI-TCRA

    Plasmid

    #131035

    Purpose

    Expresses OT-I TCRA

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pc3.1-CAGGS-ASAP3-mCyRFP3

    Plasmid

    #193323

    Purpose

    A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3

    Depositor

    Michael Lin

    Article

    Kim et al Sci Rep. 2022 Mar 7;12(1):3678. doi: 10.

    Insert

    ASAP3-R3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    caggs

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

14. pLKO.1-shQKI-3373

    Plasmid

    #115456

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shQKI-3373 (QKI Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT_TO_FlagHA_PRKRA

    Plasmid

    #106111

    Purpose

    Inducible gene expression of FlagHA-PRKRA

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pV1326

    Plasmid

    #111435

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD-MBP-His-SFP

    Plasmid

    #141141

    Purpose

    Expression of 4'-phosphopantetheinyl transferase gene

    Depositor

    New England Biolabs

    Insert

    4'-phosphopantetheinyl transferase
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pC019 - LwCas13a from Leptotrichia wadei

    Plasmid

    #91909

    Purpose

    Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a and guide expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA3.1-Nluc

    Plasmid

    #180501

    Purpose

    Mammalian expression of Nluc

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Nluc
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Hs.KRAS4b G12V

    Plasmid

    #83132

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12V mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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