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pCDNA5/FRT/TO_H2B_HaloTag7

Plasmid

#169329

Purpose

Expression of H2B-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 (H2BC21 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-NL1(-)

Plasmid

#15260

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 (Nlgn1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHluorin

Plasmid

#70113

Purpose

Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHluorin (Cd63 Mouse, Aequorea victoria)

Tags

HA-Tag and pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-CKAR2

Plasmid

#236096

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

ExRai-CKAR2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-NES

Plasmid

#199545

Purpose

expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

NES and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-mutTornado-split-nLuc

Plasmid

#212707

Purpose

Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

mutTornado-CVB3-split-nLuc

Mutation

Deleted the partial 3'ribozyme sequence in T…

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCGN-MEF2D

Plasmid

#32963

Purpose

For mammalian expression of mouse MEF2D with an HA tag

Depositor

Ron Prywes

Insert

MEF2D (Mef2d Mouse)

Tags

HA

Expression

Mammalian

Mutation

E287G

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD3

Plasmid

#153118

Purpose

Expression of Flag tagged human FOXD3 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD3 (FOXD3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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JB28

Plasmid

#128900

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab5AQL-BirA*-HA-MAO (RAB5A Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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PB-CAG-mRuby3-Gal8-P2A-Zeo

Plasmid

#150815

Purpose

Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:

Insert

mRuby3-Gal8-P2A-Zeo (LGALS8 Human, Synthetic)

Tags

Gal8 is fused to the c-terminus of mRuby3

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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