169,365 results

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pGYL1_pPromALB_FireflyLuc

Plasmid

#220282

Purpose

Firefly luciferase with minimal Albumin promoter without enhancer; enhancer of interest can be cloned into MCS

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Type

Empty backbone

Use

Luciferase

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF1498

Plasmid

#142806

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RBL1 (RBL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-GFP

Plasmid

#159654

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLARRYv2-mScarlet

Plasmid

#233210

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with mScarlet marker

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-2xFlag

Plasmid

#102931

Purpose

Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

mRFP-2xFlag

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pHelper_ShCAST_sgRNA

Plasmid

#127921

Purpose

Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Exo70

Plasmid

#53761

Purpose

pEGFP-C3 plasmid that expresses Exo70

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Exo70 (EXOC7 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV_CK8e_Cas9_miniPA

Plasmid

#226141

Purpose

AAV construct for HITI insert with CK8e promoter and SpCas9

Depositor

Christopher Nelson

Article

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Insert

Cas9

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CK8e

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iGlucoSnFR2

Plasmid

#244091

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-4

Plasmid

#172770

Purpose

Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-4

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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