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FKBP-EGFP-CLC

Plasmid

#193567

Purpose

Expresses human Clathrin Light Chain B tagged with FKBP and mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

FKBP and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CLC-FKBP-EGFP

Plasmid

#193572

Purpose

Expresses human Clathrin Light Chain B tagged with FKBP and mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

FKBP and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-CLC-SNAP

Plasmid

#193580

Purpose

Expresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

SNAP-tag and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-ShadowY-CLC

Plasmid

#193552

Purpose

Expresses mouse Clathrin Light Chain A tagged with mEGFP and ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain A (Clta Mouse)

Tags

ShadowY and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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ShadowY-CLC

Plasmid

#193560

Purpose

Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

ShadowY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CLC-ShadowY

Plasmid

#193561

Purpose

Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

ShadowY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP6-C93A-C96A_rpoA-U

Plasmid

#190991

Purpose

Test the U-to-C editing activity of KP6:C93A/C96A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Mutation

DYW-KP:C93A/C96A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP3-C93A-C96A_rpoA-U

Plasmid

#190982

Purpose

Test the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Mammalian

Mutation

DYW-KP:C93A/C96A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP6-C93A-C96A_rpoA-C

Plasmid

#190990

Purpose

Test the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Mutation

DYW-KP:C93A/C96A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP2-C93A-C96A_rpoA-U

Plasmid

#190978

Purpose

Test the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Mammalian

Mutation

DYW-KP:C93A/C96A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EK0344 SFFV-mCherry-SG(Bdnf.s1)SG-Cre (FLP-IN)

Plasmid

#191158

Purpose

Expression of a sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, Cre output; 90 bp long

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1:Cre

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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HA-dora[B]_rescue_construct

Plasmid

#190638

Purpose

Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-dora[A]_rescue_construct

Plasmid

#190640

Purpose

Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_F3194S

Plasmid

#187140

Purpose

mammalian cell expression of FLAG-HUWE1 F3194S

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

F3194S

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_H669Q

Plasmid

#187138

Purpose

mammalian cell expression of 3xFLAG-HUWE1 H669Q

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

H669Q

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-Mcl1_deltaTM

Plasmid

#187118

Purpose

insect cell expression of Mcl1-deltaTM

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

MCL-1 (BCL2 Human)

Tags

Strep

Expression

Insect

Mutation

delta328-350

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-DDIT4_delta8

Plasmid

#187117

Purpose

insect cell expression of DDIT4-delta1-8

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

DDIT4 (DDIT4 Human)

Tags

Strep

Expression

Insect

Mutation

delta1-8

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-HUWE1-HECTonly

Plasmid

#187119

Purpose

insect cell expression of HUWE1 HECT domain

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

Strep

Expression

Insect

Mutation

delta1-3992

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Daxx KO sgRNA

Plasmid

#186939

Purpose

Daxx KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Daxx KO sgRNA (Daxx Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pODN-mNG-CfANLN

Plasmid

#183837

Purpose

Repair template for the N-terminal tagging of anillin with mNeonGreen in canine cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN homology arms with mNeonGreen-linker

Use

CRISPR; Donor template

Tags

mNeonGreen-linker

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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