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pPBpuro-UbC-4493NES(Booster-PKA)

Plasmid

#184711

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2-hspDRv7_scGstlt

Plasmid

#108870

Purpose

scGESTALT V7 lineage barcode for zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Insert

scGESTALT lineage tracing target V7

Use

Tol2 integration into zebrafish genome

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEGFP-CaURA3

Plasmid

#44872

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomRuby2-Kan

Plasmid

#44953

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby2

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-BsaI_pJMP2776

Plasmid

#208890

Purpose

Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAureus-TnCAM

Plasmid

#199807

Purpose

vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

Depositor

Steve Salipante

Article

Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

Insert

transposon cassette

Expression

Bacterial

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Tg (mpx:mCherry-2A-Rac2D57N)

Plasmid

#58934

Purpose

expresses dominant negative rac2 tagged with mcherry in neutrophils

Depositor

Anna Huttenlocher

Article

Deng et al Dev Cell. 2011 Oct 18;21(4):735-45. doi:

Insert

mcherry-2a-Rac2D57N (rac2 Zebrafish)

Tags

mcherry2a

Mutation

changed Aspartate 57 to Asparagine

Promoter

mpx

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pHnCBS1D

Plasmid

#52065

Purpose

expresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducible

Depositor

David Savage

Article

Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

Insert

CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D

Expression

Bacterial

Available Since

April 4, 2014

Availability

Academic Institutions and Nonprofits only

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pSR43.6r

Plasmid

#63197

Purpose

Expresses CcaS, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pmRNA-iSyn-EGFP

Plasmid

#225907

Purpose

In vitro transcription of cap-independently (synthetic IRES) translated mRNA encoding EGFP.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

PABP motif
HRV-B3-derived synthetic IRES
EGFP
HBA1 UTR

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK622

Plasmid

#180592

Purpose

tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

Depositor

Jeffrey Barrick

Article

Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

Inserts

tdk
kanR

Use

Synthetic Biology

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJUMP22x-1A(sfGFP)

Plasmid

#126969

Purpose

Level 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTlpA39-Wasabi

Plasmid

#86116

Purpose

mWasabi reporter gene gated by TlpA39

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA39
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A, D135V, A217V, L236F

Promoter

pTlpA and pTlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGNW2

Plasmid

#122086

Purpose

suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDCC6

Plasmid

#59985

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Inserts

Cas9
U6-2>sgRNA

Use

CRISPR

Tags

3xFLAG

Promoter

U6-96Ab and hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pNO286-3

Plasmid

#107746

Purpose

Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

Depositor

Jeffrey Tabor

Article

Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Mutation

Deleted AA 223 and 512

Promoter

J23106 and J23108

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomRuby2-SpHis5

Plasmid

#44858

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby2

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pTlpA36-Wasabi

Plasmid

#86115

Purpose

mWasabi reporter gene gated by TlpA36

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA36
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A, P60L, D135V, K187R, K202I, L208Q

Promoter

pTlpA and pTlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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TS181_pETNKI_his3C_pA-Dam

Plasmid

#200721

Purpose

producing pA-DamID methylation protein in bacteria

Depositor

Bas van Steensel

Article

van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

Insert

His3C-pA-Dam

Tags

His

Expression

Bacterial

Mutation

codon optimized

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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HRE-UnaG

Plasmid

#201710

Purpose

Hypoxia responsive promoter driving UnaG fluorescent proteins

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Promoter

HRE-mCMV

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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