We narrowed to 17,223 results for: EMB
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Plasmid#207774PurposeDonor template for Blast-2A-miniTurbo insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorInsertLMNB1 Homology Arms flanking a Blast-miniTurbo Cassette (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Solo-mScarlet-ACTB
Plasmid#207750PurposeDonor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorInsertACTB Homology Arms flanking a mScarlet Tag (ACTB Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Solo-mNeon-ACTB
Plasmid#207749PurposeDonor template for mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorInsertACTB Homology Arms flanking a mNeon Tag (ACTB Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pb-miRE-tre-3xFLAG-hnRNPU
Plasmid#196087PurposeExpresses mouse hnRNPU with a 3xFLAG tag in mammalian cellsDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆sap-NLS-3XFLAG-V5
Plasmid#196091PurposeExpresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
TagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 31-65 (SAP domain) of mouse S…PromoterCAGGSAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆rrm-NLS-3XFLAG-V5
Plasmid#196093PurposeExpresses mouse SAFB lacking the rrm domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
TagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 428-506 (RRM) of mouse SAFB p…PromoterCAGGSAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV Flag-LRRK2 K17A/K18A/R1441G
Plasmid#186013PurposeFlag-LRRK2 K17A/K18A/R1441GDepositorInsertFlag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)
ExpressionMammalianMutationSee depositor's comments below.Available SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
mKate2-DrCavin1a in pT3TS-Dest
Plasmid#194294PurposeIn vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAIDepositorInsertcavin1a (cavin1a Zebrafish)
UseIn vitro transcription of mrnaAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-HsNRF2 (NFE2L2)
Plasmid#194302PurposeMultisite gateway entry clone for expression of human NRF2 (NFE2L2) with fusion tag at the N-terminus. Parton lab clone KRSDepositorInsertNFE2L2 (NFE2L2 Human)
UseMultisite gateway entry vectorAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-HsGIGYF2_1-718_AI
Plasmid#148960PurposeMammalian Expression of HsGIGYF2_1-718DepositorInsertHsGIGYF2_1-718 (GIGYF2 Human)
ExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_1-718_AI
Plasmid#148948PurposeMammalian Expression of HsGIGYF2_1-718DepositorInsertHsGIGYF2_1-718 (GIGYF2 Human)
ExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_1-718-YFWFtoAAAA_AI
Plasmid#148950PurposeMammalian Expression of HsGIGYF2_1-718-YFWFtoAAAADepositorInsertHsGIGYF2_1-718-YFWFtoAAAA (GIGYF2 Human)
ExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_280-310_AI
Plasmid#148951PurposeMammalian Expression of HsGIGYF2_280-310DepositorInsertHsGIGYF2_280-310 (GIGYF2 Human)
ExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2-F300AF306A_AI
Plasmid#148952PurposeMammalian Expression of HsGIGYF2-F300AF306ADepositorInsertHsGIGYF2-F300AF306A (GIGYF2 Human)
ExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2-W288A_AI
Plasmid#148953PurposeMammalian Expression of HsGIGYF2-W288ADepositorInsertHsGIGYF2-W288A (GIGYF2 Human)
ExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2-W288AF300AF306A_AI
Plasmid#148954PurposeMammalian Expression of HsGIGYF2-W288AF300AF306ADepositorInsertHsGIGYF2-W288AF300AF306A (GIGYF2 Human)
ExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-MBP-IST1 N-terminal domain
Plasmid#184805PurposeBacterial expression of IST1 N-terminal domainDepositorAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATX3-D1
Plasmid#184246PurposeRecombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertAtaxin-3 Josephin domain and UIM1 (ATXN3 Human)
Tags6x His-Tag and TEV cleavage siteExpressionBacterialMutationPremature stop codon N263XPromoterT7 promoterAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-MBP-CHMP1B, S2C
Plasmid#184803PurposeBacterial expression of CHMP1B (S2C mutation)DepositorAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22UbGfpnE4PCh(#301)
Plasmid#184066Purposecyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenicDepositorInserteGFP-nls-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)MutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only