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pGEX-6P-1 Caenorhabditis elegans CNNM5 CBS-pair domain

Plasmid

#166434

Purpose

Express pGEX-6P-1 Caenorhabditis elegans CNNM5 CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Caenorhabditis elegans CNNM5 CBS-pair domain

Tags

GST

Expression

Bacterial

Promoter

Tac, LacI, Amp

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Caenorhabditis elegans CNNM1 CBS-pair domain

Plasmid

#166508

Purpose

Express pGEX-6P-1 Caenorhabditis elegans CNNM1 CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Caenorhabditis elegans CNNM1 CBS-pair domain

Tags

GST

Expression

Bacterial

Promoter

Tac, LacI, Amp

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Caenorhabditis elegans CNNM3 CBS-pair domain

Plasmid

#166510

Purpose

Express pGEX-6P-1 Caenorhabditis elegans CNNM3 CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Caenorhabditis elegans CNNM3 CBS-pair domain

Tags

GST

Expression

Bacterial

Promoter

Tac, LacI, Amp

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Caenorhabditis elegans CNNM4 CBS-pair domain

Plasmid

#166511

Purpose

Express pGEX-6P-1 Caenorhabditis elegans CNNM4 CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Caenorhabditis elegans CNNM4 CBS-pair domain

Tags

GST

Expression

Bacterial

Promoter

Tac, LacI, Amp

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k

Plasmid

#127403

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon, W340A,…

Promoter

CMV

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k

Plasmid

#127407

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, K305H,…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k

Plasmid

#127401

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon, W340A,…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JW776

Plasmid

#64817

Purpose

BiFC rCUC2

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

N-terminal domain Firefly

Mutation

rCUC2

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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JW777

Plasmid

#64818

Purpose

BiFC CUC3

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC3 (CUC3 Mustard Weed)

Tags

C-terminal domain Firefly

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pIR197

Plasmid

#64819

Purpose

BiFC rCUC2

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

C-terminal domain Firefly

Mutation

rCUC2

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (5'GAC)

Plasmid

#170127

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'GAC)

Plasmid

#170129

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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PB_iETV2_P2A_GFP_Puro

Plasmid

#168805

Purpose

PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ETV2 (ETV2 Synthetic, Human)

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-TBK1-gRNA (PX459)

Plasmid

#221550

Purpose

Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human TBK1 (TBK1 Human)

Expression

Mammalian

Promoter

u6

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-TFE3

Plasmid

#38120

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFE3 (TFE3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-GCaMP6s

Plasmid

#59531

Purpose

Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
GCaMP6s

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pDblet

Plasmid

#8848

Depositor

Joel Huberman

Article

Brun et al Gene. 1995 Oct 16. 164(1):173-7.

Insert

ARS doublet

Use

Yeast cloning vector

Expression

Yeast

Mutation

This vector contains, between AatII sites, a dire…

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

Plasmid

#170229

Purpose

Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Mutation

Q64L

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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px459-TDP-43 sgRNA1

Plasmid

#133762

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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px459-TDP 43 sgRNA2

Plasmid

#133763

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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