We narrowed to 8,594 results for: Set

1. pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG

   Plasmid

   #85225

   Purpose

   Expresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

   Depositor

   John Naughton

   Inserts

   Optimized G Protein
   Enhanced GFP
   TVA (CD320 Chicken)
   

   Use

   AAV and Cre/Lox

   Promoter

   hSyn

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEED-mScarlet3

   Plasmid

   #219640

   Purpose

   Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDZ416 (24PP7SL loxP-Kan-loxP)

   Plasmid

   #45163

   Depositor

   Robert Singer, Daniel Zenklusen

   Article

   Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

   Insert

   24xPP7 Stem Loop Cassette
   

   Use

   Cre/Lox

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHelp

   Plasmid

   #227583

   Purpose

   Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   guide RNA scaffold
   

   Use

   Cloning vector

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEED-ALFA:HA

   Plasmid

   #219646

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   ALFA:HA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pALOD4

   Plasmid

   #111026

   Purpose

   Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

   Depositor

   Arun Radhakrishnan

   Article

   Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

   Insert

   Domain 4 of Anthrolysin O
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: S404C, C472A

   Promoter

   T7

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2ST

   Plasmid

   #59384

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-dfrA
   

   Use

   Template

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. EC2_1_dCas9_sgRNA

   Plasmid

   #163710

   Purpose

   Yeast low copy plasmid with dCas9 and sgRNA expression cassette

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   dCas9
   

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSP618

   Plasmid

   #169235

   Purpose

   Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

   Depositor

   William Greenleaf

   Article

   Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

   Insert

   mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEED-sfGFP:ALFA

    Plasmid

    #219642

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP:ALFA SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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