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pBbdCas9S(-10TC)

Plasmid

#149581

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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P315-FBP_10

Plasmid

#139701

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing CPH and Npga

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pUBQ1:NnH3H:tNos, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-λN-βLac

Plasmid

#162039

Purpose

Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)

Depositor

Karen Polizzi

Article

Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199

Insert

λN domain of the λ phage and a β-lactamase fusion protein

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAflaB

Plasmid

#149642

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAmreB

Plasmid

#149653

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_arr2

Plasmid

#149639

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab3aa

Plasmid

#109703

Purpose

Multisite gateway vector for 3' tagging with rab3aa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab3aa (rab3aa Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab5b

Plasmid

#109718

Purpose

Multisite gateway vector for 3' tagging with rab5b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5b (rab5b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab7b

Plasmid

#109724

Purpose

Multisite gateway vector for 3' tagging with rab7b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab7b (rab7b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab11al

Plasmid

#109663

Purpose

Multisite gateway vector for 3' tagging with rab11al

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11al (rab11al Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9K_arr2

Plasmid

#149570

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR4_FLAG/HA_EWSR1_His6

Plasmid

#26364

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

EWSR1 (EWSR1 Human)

Use

Gateway Cloning

Tags

FLAG/HA and His6

Mutation

delta 657 (stop codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
p3E-rab6a

Plasmid

#109720

Purpose

Multisite gateway vector for 3' tagging with rab6a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6a (rab6a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab6ba

Plasmid

#109721

Purpose

Multisite gateway vector for 3' tagging with rab6ba

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6ba (rab6ba Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab6bb

Plasmid

#109722

Purpose

Multisite gateway vector for 3' tagging with rab6bb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6bb (rab6bb Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pet28bZFCNGA5

Plasmid

#69858

Purpose

His tagged protein for antibody production screening

Depositor

Gary Matthews

Article

Khan et al Neuroscience. 2010 Jan 13;165(1):79-89.

Insert

ZFCNGA5, 3' end only with native stop (cnga5 Zebrafish)

Tags

His

Expression

Bacterial

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-Pacsin2

Plasmid

#109587

Purpose

Multisite gateway vector for 3' tagging with Pacsin2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Pacsin2 (pacsin2 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab33ba

Plasmid

#109692

Purpose

Multisite gateway vector for 3' tagging with rab33ba

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab33ba (rab33ba Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pME-CD44a

Plasmid

#109575

Purpose

Multisite gateway vector for 5' tagging with CD44a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CD44a (cd44a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Cav1-miniSOG

Plasmid

#67667

Purpose

Mammalian expression vector driving dog Caveolin1-minSOG

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

Dog Cav1-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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