We narrowed to 1,480 results for: asl

1. pBbS8k-RFP_E83K

   Plasmid

   #109412

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   E83K repA mutation to increase copy number

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBbS8k-RFP_R46W

   Plasmid

   #109415

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   R46W repA mutation to increase copy number

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. YIplac211-ENT5-HaloTag

   Plasmid

   #177102

   Purpose

   Expresses Ent5-HaloTag in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   ENT5 (ENT5 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKS1

   Plasmid

   #24636

   Depositor

   Jay Keasling

   Article

   Steen et al Nature. 2010 Jan 28;463(7280):559-62.

   Insert

   'tesA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

5. YIplac211-SYS1-msGFPx3

   Plasmid

   #177112

   Purpose

   Expresses Sys1-msGFP3 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   SYS1 (SYS1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NCVB-ssGrl1-ESCargo*

   Plasmid

   #140167

   Purpose

   Expresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophila

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   ssGrl1-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   MTT1

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YIplac204-pOst1-ESCargo

   Plasmid

   #115420

   Purpose

   Expresses a regulatable secretory cargo for yeast at the TRP1 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBbS8k-RFP_E93G

   Plasmid

   #109411

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   E93G repA mutation to increase copy number

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJAT13araE

   Plasmid

   #18987

   Depositor

   Jay Keasling

   Article

   Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

   Insert

   araE (araE E.coli)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pJAT13

    Plasmid

    #18986

    Depositor

    Jay Keasling

    Article

    Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

    Insert

    promoter CP13
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pBbS8k-RFP_R43W_E93G

    Plasmid

    #109406

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    R43W and E93G repA mutations to increase copy num…

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAK02

    Plasmid

    #19001

    Depositor

    Jay Keasling

    Article

    Khlebnikov et al J Bacteriol. 2000 Dec . 182(24):7029-34.

    Insert

    arabinose transporter araE under control of Ptac (araE E. coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pBbS8k-RFP_R43W

    Plasmid

    #109416

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    R43W repA mutation to increase copy number

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YIplac211-msGFP2-STE13

    Plasmid

    #177106

    Purpose

    Expresses msGFP2-Ste13 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    STE13 (STE13 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YIplac204-pOst1-ESCargo*

    Plasmid

    #115427

    Purpose

    Expresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    pOst1-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pY1

    Plasmid

    #50597

    Purpose

    Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,

    Depositor

    Jay Keasling

    Article

    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

    Type

    Empty backbone

    Use

    Synthetic Biology; Empty vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLacUV5

    Available since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pMGT1

    Plasmid

    #109404

    Purpose

    Tetracycline resistance reporter of ChnR activation

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    Tetracycline resistance reporter of ChnR activation
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YIplac211-STV1-iGFP

    Plasmid

    #177111

    Purpose

    Expresses Stv1-iGFP in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    STV1 (STV1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSec7-Halox2

    Plasmid

    #115425

    Purpose

    Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    Sec7-Halox2
    

    Use

    Tags

    HaloTag

    Expression

    Yeast

    Mutation

    Promoter

    Endogenous

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbS8k-RFP_R46Q

    Plasmid

    #109414

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    R46Q repA mutation to increase copy number

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. YIplac211-vps10-104

    Plasmid

    #115426

    Purpose

    Generate the vps10-104 allele via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    vps10-104
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    domain 1 deletion

    Promoter

    Endogenous

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBbS8k-RFP_N99D

    Plasmid

    #109410

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    N99D repA mutation to increase copy number

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUASt-ssBiP-ESCargo

    Plasmid

    #140166

    Purpose

    Expresses a regulatable secretory cargo for Drosophila melanogaster

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hs70

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSec7-iGFPx6

    Plasmid

    #115424

    Purpose

    Express Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    Sec7-iGFPx6
    

    Use

    Tags

    iGFP

    Expression

    Yeast

    Mutation

    Promoter

    Endogenous

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBAD18HMGR

    Plasmid

    #19041

    Depositor

    Jay Keasling

    Article

    Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

    Insert

    truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (HMG1 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pDL12c

    Plasmid

    #17835

    Depositor

    Jay Keasling

    Article

    Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

    Insert

    gpdA promoter, lacZ, argB_1626,
    

    Use

    Synthetic Biology; Aspergillus nidulans expression

    Tags

    Expression

    Mutation

    The argB_1626 mutation consists of two sequential…

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

27. YIplac211-iGFP-DRS2

    Plasmid

    #177104

    Purpose

    Expresses iGFP-Drs2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    DRS2 (DRS2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. YIplac211-AUR1-msGFP2

    Plasmid

    #177101

    Purpose

    Expresses Aur1-msGFP2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    AUR1 (AUR1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. YIplac211-RBD2-6xmGFP

    Plasmid

    #177109

    Purpose

    Expresses Rbd2-6xmEGFP in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    RBD2 (RBD2 Budding Yeast)
    

    Use

    Tags

    6xmEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. YIplac211-SEC2-msGFPx3

    Plasmid

    #177110

    Purpose

    Expresses Sec2-msGFPx3 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    SEC2 (SEC2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBbS8k-RFP_K102E_E115K

    Plasmid

    #109405

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    K102E and E115K repA mutations to increase copy n…

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBbS8k-RFP_N99K

    Plasmid

    #109409

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    N99K repA mutation to increase copy number

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBbS8k-RFP_K102E

    Plasmid

    #109408

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    K102E repA mutation to increase copy number

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBbS8k-RFP_E115K

    Plasmid

    #109407

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    E115K repA mutation to increase copy number

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBbS8k-RFP_M78I

    Plasmid

    #109413

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    M78I repA mutation to increase copy number

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDL11c

    Plasmid

    #17833

    Depositor

    Jay Keasling

    Article

    Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

    Insert

    alcA promoter, lacZ, argB_1626,
    

    Use

    Synthetic Biology; Aspergillus nidulans expression

    Tags

    Expression

    Mutation

    The argB_1626 mutation consists of two sequential…

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

37. pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

    Plasmid

    #35152

    Depositor

    Jay Keasling, Taek Soon Lee

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    R364S in Mevalonate Kinase

    Promoter

    LacUV5, pTrc

    Available since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTrc-trGPPS(CO)-LS

    Plasmid

    #50603

    Purpose

    pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

    Depositor

    Jay Keasling

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Inserts

    Limonene Synthase
    ApR
    LacIq
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    truncated codon optimized geranylpyrophosphate sy…

    Promoter

    Trc

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPS22 (pET23a-Cas10/Csm/csm2H6N)

    Plasmid

    #70038

    Purpose

    Bacterial expression of the S. epidermidis Cas10·Csm complex

    Depositor

    Luciano Marraffini

    Article

    Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

    Insert

    Cas10/Csm complex
    

    Use

    Tags

    6xHIS on N terminus of Csm2

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBbA5c-MevT-MBIS

    Plasmid

    #35150

    Depositor

    Jay Keasling, Taek Soon Lee

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5,

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pKS17

    Plasmid

    #24628

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    pdc, adhB, atfA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEF-pIgH-ESCargo(FTV)

    Plasmid

    #140160

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

    Plasmid

    #140168

    Purpose

    Expresses a regulatable secretory cargo for Tetrahymena thermophila

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    MTT1

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pEF-pIgH-ESCargo(FTV,Crimson)

    Plasmid

    #140162

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pESC-URA::CYP71AV1/CPR

    Plasmid

    #20118

    Depositor

    Jay Keasling

    Article

    Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

    Insert

    amorphadiene oxidase and p450 reductase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 2, 2009

    Availability

    Academic Institutions and Nonprofits only

46. pCI-HA-ERK5-FL

    Plasmid

    #31817

    Depositor

    Astar Winoto

    Article

    Kasler et al Mol Cell Biol. 2000 Nov;20(22):8382-9.

    Insert

    Erk5 (Mapk7 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

47. pEF-pIgH-ESCargo*

    Plasmid

    #140159

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pEF-pIgH-ESCargo(FTV,Orange)

    Plasmid

    #140161

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. YIplac211-VPS8-HaloTagx2

    Plasmid

    #177113

    Purpose

    Expresses Vps8-HaloTagx2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    VPS8 (VPS8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pconst #5-RFP

    Plasmid

    #81079

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23110

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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