We narrowed to 140 results for: Cas3

1. D. vulgaris Cas5c/pHMGWA

   Plasmid

   #81182

   Purpose

   Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

   Insert

   Cas5c
   

   Use

   CRISPR

   Tags

   6xHis - MBP - TEV protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. D. vulgaris Cas8c/pHMGWA

   Plasmid

   #81183

   Purpose

   Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

   Insert

   Cas8c
   

   Use

   CRISPR

   Tags

   6xHis - MBP - TEV protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b_TypeIC_acrIF2_invivo

   Plasmid

   #196402

   Purpose

   expresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assay

   Depositor

   David Taylor

   Article

   O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

   Insert

   anti-CRISPR IF2 protein (orf30 P. aeruginosa)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b_TypeIC_acrIC4_invivo

   Plasmid

   #196403

   Purpose

   expresses acrIC4 protein targeting CRISPR Cascade Type IC for in vivo assay

   Depositor

   David Taylor

   Article

   O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

   Insert

   anti-CRISPR IC4 protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA

   Plasmid

   #81185

   Purpose

   Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c

   Depositor

   Jennifer Doudna

   Article

   Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

   Inserts

   Cas5c
   Cas8c
   Cas7
   

   Use

   CRISPR

   Tags

   6xHis - MBP - TEV protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCsy3-VPR-Csy4

   Plasmid

   #153943

   Purpose

   Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy3-VPR and Csy4 in mammalian cells

   Depositor

   Zhou Songyang

   Article

   Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

   Insert

   Csy3-VPR, Csy4
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCsy1-Csy2

   Plasmid

   #153942

   Purpose

   Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy1 and Csy2 in mammalian cells

   Depositor

   Zhou Songyang

   Article

   Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

   Insert

   Csy1, Csy2
   

   Use

   AAV and CRISPR

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB758

   Plasmid

   #210033

   Purpose

   Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas7-P2A-Cas5-T2A-Cas8
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-Flag-LmoCas6

   Plasmid

   #126485

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-Flag-LmoCas8b2

    Plasmid

    #126482

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas8b2
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-Flag-LmoCas7

    Plasmid

    #126483

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas7
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-Flag-LmoCas5

    Plasmid

    #126484

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas5
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-LmoCas8b2

    Plasmid

    #126488

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas8b2 driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas8b2
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-LmoCas7

    Plasmid

    #126489

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas7 driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas7
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-LmoCas5

    Plasmid

    #126490

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas5
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-LmoCas6

    Plasmid

    #126491

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-Flag-EcoCas6

    Plasmid

    #106274

    Purpose

    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasE
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-LmoCas6-KRAB-2a-BlastR

    Plasmid

    #126492

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6-KRAB-2a-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-Flag-EcoCas8e

    Plasmid

    #106270

    Purpose

    Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasA
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-Flag-EcoCse2

    Plasmid

    #106271

    Purpose

    Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasB
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge