We narrowed to 172 results for: LPP
-
Plasmid#85496PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
VN1263 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP1_Ddcm_WeaKRBS_TIR51_KanR
Plasmid#235115PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterLtetO and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDule2-Tet2.0
Plasmid#85497PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule2-IBBN (G2)
Plasmid#85501PurposePlasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertIBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32G L65E F108W Q109M D158S R162KPromoterlpp (constitutive)Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule-IBBN (G2)
Plasmid#85500PurposePlasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertIBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32G L65E F108W Q109M D158S R162KPromoterlpp (constitutive)Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
VN1287 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP2_Ddcm_WeaKRBS_TIR51_KanR
Plasmid#235116PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterLtetO and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN1262 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3_Ddcm_WeaKRBS_TIR51_KanR
Plasmid#235117PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterLtetO and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN999 pB2Hws_v9_cI-Asf1B+rpoA-IP3mut3A
Plasmid#235104PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterlacUV5 and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN991 pB2Hws_v9_cI-Asf1B+rpoA-IP1
Plasmid#235105PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterlacUV5 and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN1043 pB2Hws_v9_cI-Asf1B+rpoA-IP2
Plasmid#235106PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterlacUV5 and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN990 pB2Hws_v9_cI-Asf1B+rpoA-IP3
Plasmid#235107PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterlacUV5 and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN1335 pB2Hws_v9_cI-Asf1B+rpoA-IP4
Plasmid#235108PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP4.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterlacUV5 and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN1312 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3mut3A_Ddcm_eGFP-wcKanR
Plasmid#235109PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterLtetO and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN1313 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP1_Ddcm_eGFP-wcKanR
Plasmid#235110PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterLtetO and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN1314 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP2_Ddcm_eGFP-wcKanR
Plasmid#235111PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterLtetO and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN1315 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3_Ddcm_eGFP-wcKanR
Plasmid#235112PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterLtetO and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN1264 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3mut3A_Ddcm_WeaKRBS_TIR51_KanR
Plasmid#235114PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterLtetO and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)
Plasmid#174515PurposeRecoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)DepositorInsertsMm PylS
MmtRNA-Pyl-opt(UGA)
ExpressionBacterialMutationASL from the serT gene (UGA anticodon) and recodedPromoterGlnS and lppAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDule-3-nitroTyrosine (5B)
Plasmid#85498PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only