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Plasmid#208073Purposeexpression of GFP-tagged hsRPA2-Ala10 in human cellsDepositorInsertRPA2 (RPA2 Human)
UseTagsGFPExpressionMammalianMutationmutations of 10 ser/thr residues in N-them domain…PromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA32-Asp8 (MSW#507)
Plasmid#208072Purposeexpression of GFP-tagged hsRPA2-Asp8 in human cellsDepositorInsertRPA2 (RPA2 Human)
UseTagsGFPExpressionMammalianMutationmutations of 8 ser and thr residues in N-term dom…PromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPa-FRB-pre mGRASP
Plasmid#73145PurposeLarge fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.DepositorInsertsHRPa-FRB-pre mGRASP
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET16b-RPA4/His14 (MSW#949)
Plasmid#208075PurposeExpression of human Replication Protein A 4 and His-tagged RPA3 in E.coliDepositorInsertRPA4, RPA14(His-tagged)
UseTags10xHis tag on RPA14ExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Synapsin sHRPa-NRX in FSW lentiviral vector
Plasmid#73147PurposeLarge fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.DepositorInsertsHRPa-NRX
UseLentiviralTags2x HA tag and Flag tagExpressionMammalianMutationPromoterSynapsinAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIRPA-Fc(DAPA)-AviTag-6xHis
Plasmid#156783PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSIRPA (SIRPA Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta
Plasmid#79913PurposepiggyBac transposon vector, Zeocin-2A-sHRPaNRX3betaDepositorInsertZeocin-t2A-sHRPbNRX3beta
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1
Plasmid#79914PurposepiggyBac transposon vector, Zeocin-2A-sHRPbNLG1DepositorInsertZeocin-t2A-splitHRP-aNLG1 (Nlgn1 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLN43 - pBait-1xMS2-7GC[chiP-5']-TtrpA
Plasmid#222404Purposearabinose-induced synthesis of a hybrid RNA containing a 5' MS2 hairpin, E. coli chiP 5'UTR insert between XmaI and HindIII sites, surrounded by a 7bp GC clamp, and followed by a 3' trpA terminator.DepositorInsert5’UTR of chiP (-98 to +12, relative to AUG) (chiP E. coli)
UseTags1xMS2 hairpin, 7GC clamp, TtrpA terminatorExpressionBacterialMutationPromoterpBADAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSS2 - pBait-1xMS2-7GC[empty]-TtrpA
Plasmid#222406Purposearabinose-induced synthesis of an RNA containing an MS2 hairpin, 7GC clamp and trpA terminator. Used as a three-hybrid control. For cloning purposes, see plasmid #222404.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgRPA1-2_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211659PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgRPA1-2 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgRPA1-3_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211660PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgRPA1-3 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgRPA1-1_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211658PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgRPA1-1 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIRPA-COMP5AP-AviTag-9xHis
Plasmid#157347PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertSIRPA (SIRPA Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28Teb1
Plasmid#28173DepositorInsertTeb1
UseTags6xhistidineExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hTipin (pc4732)
Plasmid#216862PurposeMammalian expression vector encoding GFP tagged human TipinDepositorInsertTipin (TIPIN Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hTimeless (pc4729)
Plasmid#216861PurposeMammalian expression vector encoding GFP tagged human TimelessDepositorInsertTimeless (TIMELESS Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
KBB285
Plasmid#185076PurposeRFA3-GFP fusionDepositorInsertRFA3
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB284
Plasmid#185075PurposeRFA2-GFP fusionDepositorInsertRFA2
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB329
Plasmid#185077PurposeNUP82 expression in yeastDepositorInsertNUP82
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only