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Plasmid#40731DepositorInsertAurora B FRET-sensor
UseTagsCyPet and YPetExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP
Plasmid#227012PurposeLentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domainDepositorInsertdCas9
UseCRISPR and LentiviralTags3xFLAG, SV40 NLSExpressionMutationPromoterUbCAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)
Plasmid#166415PurposeExpress pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)DepositorInsertAcytostelium subglobosum PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)
Plasmid#55218DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Histone H2B targeted Aurora B FRET sensor Kif2 substrate
Plasmid#45216DepositorInsertHistone H2B-targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
Plasmid#40571DepositorInsertAurora B FRET-sensor N-terminally fused to histone 2B
UseTagsCyPet and YPetExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)
Plasmid#55219DepositorTypeEmpty backboneUseTagsHis6ExpressionInsectMutationPromoterAvailable sinceJune 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)
Plasmid#55220DepositorTypeEmpty backboneUseTagsHis6 and MBP, Asn10ExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)
Plasmid#55221DepositorTypeEmpty backboneUseTagsStrepII and msfGFPExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)
Plasmid#55222DepositorTypeEmpty backboneUseTagsSNAPf and TwinStrepIIExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)
Plasmid#55223DepositorTypeEmpty backboneUseTagsSNAPf and TwinStrepIIExpressionInsectMutationPromoterAvailable sinceJuly 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Myr-Pink Flamindo
Plasmid#204669PurposeMammalian expression of membrane-bound Pink Flamindo for cAMP imagingDepositorArticleInsertMyr-Pink Flamindo
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-paGFP-bPAC
Plasmid#204670PurposeMammalian expression of bPAC tagged with paGFP for cAMP productionDepositorArticleInsertpaGFP-bPAC
UseLentiviralTags10xHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-Ferritin-Dronpa
Plasmid#101194PurposeExpresses a Ferritin-Dronpa fusion construct in E. coliDepositorInsertFerritin-Dronpa
UseTags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationPromoterT7Available sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-ffDronpa-F
Plasmid#101193PurposeA fast switching Dronpa variantDepositorInsertffDronpa-F
UseTags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationK45I, V60A, F173VPromoterT7Available sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHBJT017
Plasmid#225168PurposeLow copy cloning vector with same multiple cloning site as pUC19 and pSU19 (AmpR).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT024
Plasmid#225175PurposeLow copy cloning vector with same multiple cloning site as pUC18 and pSU18 (SmR).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT023
Plasmid#225174PurposeLow copy cloning vector with same multiple cloning site as pUC19 and pSU19 (SmR).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT019
Plasmid#225170PurposeLow copy cloning vector with same multiple cloning site as pUC19 and pSU19 (TetR).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT020
Plasmid#225171PurposeLow copy cloning vector with same multiple cloning site as pUC18 and pSU18 (TetR).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only