We narrowed to 18,145 results for: bon;

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L4278 hIgKLSS_hTGFb1 in pcDNA backbone

Plasmid

#244184

Purpose

Constitutive expression of human TGF-beta1 (with the human IgK light chain secretion sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

TGF-beta1 with IgK light chain secretion sequence (TGFB1 Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L4275 hIgKLSS_hTNF in pcDNA backbone

Plasmid

#244182

Purpose

Constitutive expression of human mature TNF (with the human IgK light chain secretion sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

TNF with IgK light chain secretion sequence (TNF Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLUEF1 IRES GFP (backbone)

Plasmid

#224466

Purpose

Ef1a downstream of a ubiquitous chromatin opening element drives expression of GOI and IRES GFP

Depositor

Robert Kerbel

Article

Khan et al Cancer Res. 2025 Apr 14. doi: 10.1158/00

Type

Empty backbone

Use

Lentiviral

Promoter

Ef1a

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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cASIC1 concatemer - vector backbone

Plasmid

#233132

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Type

Empty backbone

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-S1S2 [rev-backbone]

Plasmid

#229199

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-NbON10-NanoLuc

Plasmid

#198691

Purpose

Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, IgK leader sequence, NanoLuc, and twin…

Expression

Mammalian

Promoter

CMV promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2368 MTK PB puro backbone

Plasmid

#226673

Purpose

Empty MTK PB cargo backbone marked with puromycin resistance.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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3'-com-gRNA GG backbone (MOBE1-4)

Plasmid

#219947

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-com-3'end (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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3'MS2-gRNA GG backbone (MOBE1-2)

Plasmid

#219948

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-3'end (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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Immune checkpoint (library 2) Backbone

Plasmid

#192363

Purpose

Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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T cell activation (library 1) Backbone

Plasmid

#192364

Purpose

Plasmid vector for in vitro Jurkat activation screening (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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in-frame ampicillin PKAKTR backbone

Plasmid

#177197

Purpose

PKAKTR mutagenesis template with in-frame ampicillin site

Depositor

Markus Covert

Article

Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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lenti sgRNA(MS2)_zeo backbone

Plasmid

#61427

Purpose

3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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L4868 hVEGFA-165 in pcDNA backbone

Plasmid

#244179

Purpose

Constitutive expression of human VEGFA-165 isoform

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

VEGFA-165 (VEGFA Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXR003: CasRx gRNA cloning backbone

Plasmid

#109053

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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lenti sgRNA(MS2)_puro backbone

Plasmid

#73795

Purpose

3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-A0201-SABR-Backbone

Plasmid

#119050

Purpose

3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

MND

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-tmpknot-epegRNA-puro backbone

Plasmid

#214089

Purpose

Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6, EF1a

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pNP2321 (Cloning backbone, bRNA + recombinase]

Plasmid

#247249

Purpose

Cloning backbone for cloning bridge recombinase and bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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RNA motif plasmid cloning backbone

Plasmid

#107253

Purpose

RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

RNA motif plasmid cloning backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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