We narrowed to 593 results for: pFAST

1. pFastBac GST-Plk4

   Plasmid

   #80264

   Purpose

   baculovirus creation plasmid for GST-Plk4

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFastBacDual with LFn + FlaAAA

   Plasmid

   #84867

   Purpose

   Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   FlaAAA
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Insect

   Mutation

   3 C-terminal leucines have been replaced by alani…

   Promoter

   Polyhedrin

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBac mph1 flag

   Plasmid

   #1985

   Depositor

   Robert Kingston

   Article

   Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

   Insert

   mPh1 (Phc1 Mouse)
   

   Use

   Tags

   flag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pfastbac/mBmal62-447

   Plasmid

   #47335

   Purpose

   mBmal fragment cloned into pfastbac and crystallized

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   aa 62-447

   Promoter

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pFastBac flag Mel18

   Plasmid

   #1968

   Depositor

   Robert Kingston

   Article

   Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

   Insert

   Mel18 (Pcgf2 Mouse)
   

   Use

   Tags

   flag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pFastBac-CHD7 (1-1899)

   Plasmid

   #170143

   Purpose

   Expresses residues 1-1899 of CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   C-terminal truncation of residues 1900-2997

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac-CHD7 (1-1547)

   Plasmid

   #170144

   Purpose

   Expresses residues 1-1547 of CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   C-terminal truncation of residues 1548-2997

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac-CHD7 (K999R)

   Plasmid

   #170142

   Purpose

   Expresses CHD7 (K999R) in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Insect

   Mutation

   K999R

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pfastbacHTb/mClock26-384

   Plasmid

   #47336

   Purpose

   mClock fragment cloned into pfastbacHTb vector with Hexa His tag

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   His and Tev

   Expression

   Mutation

   aa 26-384

   Promoter

   PH

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pFastBacDual with LFn + PrgJ

    Plasmid

    #84869

    Purpose

    Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    PrgJ
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFAST NPC1(NTD)-3Q

    Plasmid

    #25772

    Depositor

    Johann Deisenhofer

    Article

    Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

    Insert

    npc1 (NPC1 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    aa23-252 only; N70Q N122Q N185Q

    Promoter

    Available since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pfast NPC1L1(NTD)

    Plasmid

    #37366

    Depositor

    Johann Deisenhofer

    Article

    Kwon et al PLoS One. 2011 Apr 15;6(4):e18722.

    Insert

    npc1l1 (NPC1L1 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    aa22-284only

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

13. 892 pFastBacHTb PTEN

    Plasmid

    #10938

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pFastBac-Giardia Dicer

    Plasmid

    #89146

    Purpose

    Expression of Giardia Dicer in Sf9 cells

    Depositor

    Jennifer Doudna

    Article

    MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

    Insert

    Giardia Dicer
    

    Use

    Tags

    His6-teve

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pfast NPC1(NTD)

    Plasmid

    #25773

    Depositor

    Johann Deisenhofer

    Article

    Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

    Insert

    npc1 (NPC1 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    aa 23-252 only

    Promoter

    Available since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pFastBac GST-STIL

    Plasmid

    #80265

    Purpose

    baculovirus creation plasmid for GST-STIL

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac-His6-Mps1-KD

    Plasmid

    #114064

    Purpose

    protein production

    Depositor

    Geert Kops

    Article

    Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

    Insert

    Mps1-KD (TTK Human)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Mps1 KD (D664A)

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac K-Flag M33

    Plasmid

    #1992

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    M33 (Cbx2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Kozak sequence added before ATG

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pfast bac cry1myc

    Plasmid

    #51892

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    cry1 (Cry1 Mouse)
    

    Use

    Tags

    3x Flag and myc

    Expression

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pFastBac K-M33-Flag

    Plasmid

    #1993

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    M33 (Cbx2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Kozak sequence added before ATG

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only