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Halotag-6xHp

Plasmid

#214414

Purpose

Lipid droplet localization of 6xHp of Spastin fused to Halotag

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

6x Spastin aa 43-92 (SPAST Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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CFAST-Halotag-6xHp

Plasmid

#214416

Purpose

Lipid droplet localization of CFAST fused to Halotag-6xHp

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

deletion

Use

Tags

CFAST-Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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mApple-1xHp

Plasmid

#214409

Purpose

Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

Spastin aa 43-92 (SPAST Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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prhaBAD-pA-Hia5

Plasmid

#222303

Purpose

Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

pA-Hia5

Use

Tags

6His and protein A

Expression

Bacterial

Mutation

codon optimized for bacterial expression

Promoter

rhaBAD

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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PMP34-mApple

Plasmid

#214417

Purpose

Peroxisome localization of PMP34 fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

PMP34 (SLC25A17 Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MMP8 Wt

Plasmid

#29545

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+ Flag-RAP2A

Plasmid

#128099

Purpose

Expresses RAP2A in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

RAP2A (RAP2A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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APEX2-6xHp

Plasmid

#214411

Purpose

Lipid droplet localization of APEX2-6xHp for electron microscopy

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

6x Spastin aa 43-92 (APEX2 Human)

Use

Tags

APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ Flag-RAGB99L

Plasmid

#128101

Purpose

Expresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

Ras related GTP binding B (RRAGB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Q99L (CAA to CTA)

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MMP8 (P78S)

Plasmid

#29546

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

P78S

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MMP8 (G104R)

Plasmid

#29548

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

G104R

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry

Plasmid

#208834

Purpose

Control plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFP

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K12(1)-U6-sgMap3K12(2)-hSyn1-mCherry

Plasmid

#208835

Purpose

Knockout of Map3K12 (DLK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-U6-sgMap3K13(1)-U6-sgMap3K13(2)-hSyn1-mCherry

Plasmid

#208836

Purpose

Knockout of Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PMP34-mApple-NFAST(low)

Plasmid

#214418

Purpose

Peroxisome localization of low-affinity NFAST fused to PMP34-Apple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N (SLC25A17 )

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MMP8 (E87D)

Plasmid

#29547

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E87D

Promoter

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
PCMV-Tag4A-MMP8 (E138Q)

Plasmid

#29549

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E138Q

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
Lenti-FAB(PX-LD)

Plasmid

#214422

Purpose

Detection of peroxisome-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

PMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC (SLC25A17 Human)

Use

Lentiviral

Tags

CFAST-Halo and FKBP-V5-RspAN

Expression

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-sgTSC2

Plasmid

#128098

Purpose

CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

sgRNA against human TSC2 (TSC2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K13(1)-U6-sgMap3K12(1)-hSyn1-mCherry

Plasmid

#208837

Purpose

Knockout of Map3K12 (DLK) and Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge