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pMXs-Ecat8

Plasmid

#13357

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

ES cell associated transcript 8 (Tdrd12 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-ms-c-Myc-L420P

Plasmid

#50776

Purpose

retroviral expression of mouse c-Myc L420P mutant

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

Insert

c-Myc L420P (MYC, Myc Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

L420P (does not bind to Max)

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-ms-Sox1

Plasmid

#50778

Purpose

retroviral expression of mouse Sox1

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox1 (Sox1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p5xFbx15 (-544/-527)-TK-Luc

Plasmid

#15686

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

5 x Fbx15 enhancer (Oct/Sox binding site)

Use

Luciferase

Tags

Expression

Mutation

Wild-type

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pTV-Nanog Hyg

Plasmid

#13836

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

IRES-Hyg-pA cassette flanked by Nanog homologous regions (Nanog Mouse)

Use

Beta-gal

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-hu-c-Myc-dN2

Plasmid

#50774

Purpose

retroviral expression of human c-Myc dN2 fragment

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

Insert

c-Myc -dN2 (MYC Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

N-terminal deletion, contains aa 42-439

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-hu-c-Myc-dN1

Plasmid

#50773

Purpose

retroviral expression of human c-Myc dN1 fragment

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

Insert

c-Myc -dN1 (MYC Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

N-terminal deletion, contains aa 14-439

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-hGLIS1GR

Plasmid

#59312

Purpose

Forced expression of human GLIS1GR

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

GLIS1 (GLIS1 Human)

Use

Retroviral

Tags

cGR - hormone binding domain of glucocorticoid re…

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pPyCAG-Myc-ERas-IP

Plasmid

#15690

Depositor

Shinya Yamanaka

Article

Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

Insert

ERas (Eras Mouse)

Use

Tags

Myc (6x)

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pMKO.1-puro/hFOXH1 shRNA1

Plasmid

#59303

Purpose

Knockdown of human FOXH1

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

hFOXH1 shRNA (FOXH1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pPyCAG-Nanog(L122A)-IP

Plasmid

#62992

Purpose

Expresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

Depositor

Shinya Yamanaka

Insert

Mouse Nanog L122A Mutant (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

Changed Leucine 122 (123 AA in Mouse Protein) to …

Promoter

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-EGFP-Rheb SSVM-IP

Plasmid

#13832

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb SSVM (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C181 changed to serine

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pCAG-ERas-IH

Plasmid

#15688

Depositor

Shinya Yamanaka

Article

Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

Insert

ERas (Eras Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
p5xFbx15 (-544/-527)-TK-Luc/Sox-m1

Plasmid

#15687

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

5 x mutated Fbx15 enhancer (Oct/Sox binding site)

Use

Luciferase

Tags

Expression

Mutation

Mutated in Sox binding site

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-deltaCoiledCoil-LIN-41

Plasmid

#52720

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the Coiled Coil domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 328–447

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-EGFP-Rheb-HVR HRas-IP

Plasmid

#13833

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb-HVR HRas (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminal of Rheb and hyper variable region (HVR…

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-EGFP-ERas delCAAX-IP

Plasmid

#13834

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas delCAAX (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Deletion 4 amino acids of c-terminal end (CAAX mo…

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-EGFP-ERas C220S/C222S-IP

Plasmid

#13829

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas C220S/C222S (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C220 and C222 changed to serine residues

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-hFOXH1GR

Plasmid

#59311

Purpose

Forced expression of human FOXH1GR

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

FOXH1 (FOXH1 Human)

Use

Retroviral

Tags

cGR - hormone binding domain of glucocorticoid re…

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-EGFP-ERas SSVA-IP

Plasmid

#13827

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas SSVA (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C224 changed to serine

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only