Addgene

Showing: 101 - 120 of 3333 results

Results per page:

Sort results by:

pSCALPS-FLAG-HA-mmuMettl3-LN

Plasmid

#207129

Purpose

Lentiviral plasmid to express mouse Mettl3 Lambda peptide C-ter

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Mettl3 (Mettl3 Mouse)

Use

Lentiviral

Tags

FLAG-HA and Lambda Peptide (LN)

Expression

Mutation

Promoter

SSFV

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-dCasRx-METTL3-PURO

Plasmid

#174218

Purpose

Expresses dCasRx-METTL3 in mammalian cell

Depositor

Qi Xie

Article

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

dCasRx-METTL3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pMSCV

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pShuttle-CMV-IRES-GFP

Plasmid

#128036

Purpose

Adenoviral shuttle vector with CMV promoter, coexpresses EGFP

Depositor

Oskar Laur

Type

Empty backbone

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pShuttle CMV RGECO-TnT

Plasmid

#124643

Purpose

Myofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.

Depositor

Paul Robinson, Matthew Daniels, Hugh Watkins

Article

Sparrow et al Circ Res. 2019 Feb 8. doi: 10.1161/CIRCR

Insert

RGECO-TnT (TNNT2 Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pShuttle-mRFP-gsk3 s9a

Plasmid

#24371

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

GSK3beta(S9A) (GSK3B Human)

Use

Adenoviral

Tags

mRFP

Expression

Mammalian

Mutation

S9A. Mutant version of GSK3 that lacks the inhibi…

Promoter

Available since

May 13, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8-attL1-pegRNA-attR3

Plasmid

#213051

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA-FLAG-METTL3-APPA

Plasmid

#160251

Purpose

Express catalytic inactive FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

CMV

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pShuttle-CMV-F2A-T2A

Plasmid

#62622

Purpose

AdEasy Shuttle vector modified for multicistronic expression. Contains T2A and F2A

Depositor

Zheng-Xu Wang

Insert

MCS with F2A and T2A

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8-attL1-pegRNA-attR5

Plasmid

#213053

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HH-gRNA-HDV-Shuttle-Vector

Plasmid

#169098

Purpose

Backbone plasmid to generate HH-gRNA-HDV segment

Depositor

Marianne Bronner

Article

Gandhi et al Development. 2021 Mar 9. pii: dev.193565

Insert

HH-gRNAbackbone-HDV

Use

Tags

Expression

Mammalian

Mutation

Promoter

NA

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pShuttle-CMV-GFP U6promoter

Plasmid

#128049

Purpose

Adenoviral shuttle vector for shRNA expression from U6 promoter with GFP marker

Depositor

Oskar Laur

Type

Empty backbone

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrc99S-ssDsbA-TTlight-4xDQNAT

Plasmid

#128402

Purpose

Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin light chain domain

Use

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Mutation

Inactivating E234A mutation

Promoter

trc

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ePB-PURO-FLAG-METTL3

Plasmid

#160252

Purpose

For stable integration of FLAG-METTL3 in human cells with trasposase

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pShuttle CMV RGECO-TnI

Plasmid

#124642

Purpose

Myofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination

Depositor

Paul Robinson, Matthew Daniels, Hugh Watkins

Article

Sparrow et al Circ Res. 2019 Feb 8. doi: 10.1161/CIRCR

Insert

RGECO-TnI (TNNI3 Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pF714-pET28a-Hs-FL-METTL20-NHis wt

Plasmid

#85115

Purpose

expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length METTL20 (METTL20 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only
pShuttle-CMV-GFP H1promoter

Plasmid

#128050

Purpose

Adenoviral shuttle vector for shRNA expression from H1 promoter with GFP marker

Depositor

Oskar Laur

Type

Empty backbone

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Brm BDmut-Flag pShuttle

Plasmid

#122108

Purpose

Expression of full length Brm containing point mutations in its bromodomain in mammalian cells

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brm (SMARCA2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-PIG-METTL14-R298P

Plasmid

#141119

Purpose

Retroviral overexpression of mutant METTL14 in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

METTL14 (METTL14 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Substitution - Missense, position 298, R➞P

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pShuttle AMPK gamma1 H150R

Plasmid

#15999

Depositor

Morris Birnbaum

Insert

AMPK gamma1 (Prkag1 Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Mutation

H150R

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only