We narrowed to 29,542 results for: REP

1. pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

   Plasmid

   #113884

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   pHR-CMV-TetO2

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

   Plasmid

   #84471

   Purpose

   pAAV-RAM-ChR2

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   Channelrhodopsin 2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mutation

   H134R

   Promoter

   TRE

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115267

   Purpose

   Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   LbuCas13a
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiUBC CASP1[CARD]-mSG2 (inflammasome reporter)

   Plasmid

   #228044

   Purpose

   Lentiviral stable Inflammasome speck reporter, designed from PMC9623368.

   Depositor

   Wade Harper

   Insert

   CASP1[CARD]-mSG2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTF275 (pFA6a-TEV-6xGly-2xStrep-His3MX)

   Plasmid

   #44070

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   2xStrep tag, 6xGly linker, ADH1 transcription ter…

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherry

   Plasmid

   #172640

   Purpose

   Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe-Puro-IKBalpha-mut (super repressor)

   Plasmid

   #15291

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   inhibitor of Kappa B alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S32A/S36A: “super-repressor” allele (resistant to…

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115268

   Purpose

   Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   EiCsm6
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-GFP-IKBalpha-mut (super repressor)

   Plasmid

   #15264

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKB alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Serine 32 to Alanine, Serine 36 to Alanine

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

    Plasmid

    #113886

    Purpose

    Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

    Plasmid

    #113885

    Purpose

    Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-Enh eGFP Reporter-muKC2-X11

    Plasmid

    #59235

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    Krt7-ex2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Mu Hsp68 minimal promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

    Plasmid

    #84472

    Purpose

    pAAV-RAM-ArchT

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    ArchT
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-AU1

    Plasmid

    #162111

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to StrepTagII-ProtC-AU1
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-AU1

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-FLAG

    Plasmid

    #162090

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to StrepTagII-ProtC-FLAG
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-FLAG

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-AU1

    Plasmid

    #162082

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-StrepTagII-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-StrepTagII-AU1

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ai62(TITL-tdT) Flp-in replacement vector

    Plasmid

    #61576

    Purpose

    Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    chromatin insulator flanked TRE-LSL-tdTomato
    

    Use

    Recombinase-mediated cassette exchange using flp …

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28-Strep-OPA1(253-960) (SB263)

    Plasmid

    #227612

    Purpose

    Inducible expression of Strep-tagged Opa1(253-960) in E.coli

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    OPA1 (OPA1 Human)
    

    Use

    Tags

    Twin-StrepTag

    Expression

    Bacterial

    Mutation

    contains aa 253-960 only

    Promoter

    T7

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFl Strep-Sumo-TEV human OAS1

    Plasmid

    #130895

    Purpose

    Human oligoadenylate synthase 1 (OAS1) is used to label terminal ADP-riobos

    Depositor

    Leemor Joshua-Tor

    Article

    Ando et al Mol Cell. 2019 Feb 21;73(4):845-856.e5.

    Insert

    oligoadenylate synthase 1 (OAS1 Human)
    

    Use

    Tags

    Strep_Sumo

    Expression

    Insect

    Mutation

    Promoter

    ph

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. SOX9-T2A-H2B-EGFP repair template

    Plasmid

    #167972

    Purpose

    SOX9 reporter repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    SRY-Box Transcription Factor 9 (SOX9 Human)
    

    Use

    CRISPR; Donor template

    Tags

    H2B-EGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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