Addgene

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Showing: 2361 - 2400 of 151085 results

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pLV-PB2_Lock1-I436C+S993C

Plasmid

#182879

Purpose

Lentivirus for expression of Plexin-B2 locked ring mutant

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2025 Jan 2;16(1):272. doi: 1

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Tags

Expression

Mutation

changed Isoleucine 436 to Cysteine and Serine 993…

Promoter

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-PB2_Lock2-I436C+T1051C

Plasmid

#182880

Purpose

Lentivirus for expression of Plexin-B2 locked ring mutant

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2025 Jan 2;16(1):272. doi: 1

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Tags

Expression

Mutation

changed Isoleucine 436 to Cysteine and Threonine …

Promoter

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-EGFP

Plasmid

#80605

Purpose

Mammalian expression vector that expresses HIV-1 GAG with EGFP fused to it

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

SRalpha

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pFUGW SpCas9

Plasmid

#131506

Purpose

lentiviral expression of SpCas9 under hUBC promotor

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

SpCas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUBC

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV MCS mCherry-KASH

Plasmid

#139654

Purpose

Cloning template for AAV-based ORANGE knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pFUGW mCherry-KASH

Plasmid

#131505

Purpose

mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry_KASH

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUBC

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-GW

Plasmid

#194634

Purpose

Gateway destination vector for mammalian expression of Virotrap bait constructs

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Use

Tags

PQS1

Expression

Mammalian

Mutation

Promoter

SRalpha

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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PBB-79

Plasmid

#8813

Depositor

Cynthia Wolberger

Article

Batchelor et al Science 1998 Feb 13;279(5353):1037-41.

Insert

GABPb1 (1-157) (Gabpb1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pMET7-GAG-eDHFR

Plasmid

#194635

Purpose

Mammalian expression of the eDHFR control bait in Virotrap

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Use

Tags

ecDHFR

Expression

Mammalian

Mutation

Promoter

SRalpha

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGPA1

Plasmid

#115476

Purpose

Generation of mariner-based transposon mutant libraries in Enterococcus faecium

Depositor

Willem van Schaik

Article

Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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ND4-DdCBE-right side TALE

Plasmid

#157843

Purpose

expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Cloning template vector

Plasmid

#131471

Purpose

Cloning template form ORANGE method based knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE LOX Cloning template vector

Plasmid

#139651

Purpose

Cloning template for ORANGE-CAKE conditional knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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ND6-DdCBE-right side TALE

Plasmid

#157841

Purpose

expresses right-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
G1333 DddAtox-C–dSpCas9

Plasmid

#157834

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG_EGFP-GFE3

Plasmid

#79869

Purpose

Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-GFE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Beta-actin

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG_EGFP-RandE3

Plasmid

#79870

Purpose

Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-RandE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Beta-actin

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
G1397 DddAtox-N–dSpCas9

Plasmid

#157835

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE GFP-Grin1 KI #3

Plasmid

#139659

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Rims1-GFP KI

Plasmid

#131494

Purpose

Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rims1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE GFP-Grin1 KI #2

Plasmid

#139658

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
G1333 DddAtox-N–dSpCas9

Plasmid

#157833

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMET7-GAG-HRAS

Plasmid

#80604

Purpose

Mammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

HIV-1 p55 GAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

SRalpha

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
G1397 DddAtox-C–dSpCas9

Plasmid

#157836

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE WASF1-GFP KI

Plasmid

#131502

Purpose

Endogenous tagging of WASP1/WAVE1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Wasf1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
(I) pXYL (J) P3 (SBE108)

Plasmid

#194991

Purpose

promoter of the gene xylose reductase from R. toruloides for a position P3

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pXYL (J)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Cacnb3-GFP KI

Plasmid

#139662

Purpose

Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Cacnb2-GFP KI

Plasmid

#139661

Purpose

Endogenous tagging of CaVβ2: C-terminal (amino acid position: Q655)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
(J) crtYB(K) G3 (SBE117)

Plasmid

#194996

Purpose

Rhodotorula toruloides gene phytoene synthase/lycopene cyclase (crtYB, RHTO_04605) for G3

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(J) crtYB(K)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(G) crtI (H) G2 (SBE116)

Plasmid

#194995

Purpose

Rhodotorula toruloides gene phytoene dehydrogenase (crtI, RHTO_04602) for G2

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(G) crtI (H)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(D) crtE (E)_G1 (SBE115)

Plasmid

#194994

Purpose

Rhodotorula toruloides gene geranylgeranyl diphosphate synthase (crtE, RHTO_02504) for G1

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(D) crtE (E)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4-DdCBE- left side TALE

Plasmid

#157844

Purpose

expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19-TdTwt

Plasmid

#207466

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTwt (Dntt Mouse)

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ND6-DdCBE-left side TALE

Plasmid

#157842

Purpose

expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE unc13a GFP KI

Plasmid

#131498

Purpose

Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Unc13a Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
G1333 DddAtox-N–SaKKH-Cas9(D10A)

Plasmid

#157837

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO_EGFP-GFE3

Plasmid

#79871

Purpose

For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-GFE3

Use

AAV, Cre/Lox, and Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE GFP-Actb KI #2

Plasmid

#139666

Purpose

Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pyc111s

Plasmid

#158781

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6s-RPL10a

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19-MTdTwt

Plasmid

#207460

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTwt (Dntt Mouse)

Use

Tags

10xHis-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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