We narrowed to 12,364 results for: NUC

1. pET22b RNase A

   Plasmid

   #58903

   Purpose

   Bacterial expression of bovine pancreatic ribonuclease A

   Depositor

   Ronald Raines

   Article

   delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

   Insert

   RNase A (RNASE1 Bovine)
   

   Use

   Tags

   pelB sequence

   Expression

   Bacterial

   Mutation

   See Depositor Comments below

   Promoter

   T7

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-C1-RanQ69L

   Plasmid

   #30309

   Depositor

   Jay Brenman

   Article

   Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

   Insert

   RanQ69L (RAN N/A)
   

   Use

   Tags

   FLAG-Cherry

   Expression

   Mammalian

   Mutation

   Glutamine 69 is changed to Leucine

   Promoter

   Available since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-NFIA1.1

   Plasmid

   #112698

   Purpose

   Expression of NFIA1.1 protein for transient transfection assays

   Depositor

   Richard Gronostajski

   Article

   Chaudhry et al J Biol Chem. 1999 Mar 12;274(11):7072-81

   Insert

   Nuclear Factor I A1.1 (Nfia Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57-mcGAS-LL/RK-NLS/D307A

   Plasmid

   #185459

   Purpose

   Inducible nuclear cGAS with D307A mutation

   Depositor

   Shitao Li

   Article

   Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

   Insert

   cGAS
   

   Use

   Lentiviral

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   L157R, L159K, D307A

   Promoter

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

   Plasmid

   #40132

   Purpose

   Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

   Depositor

   Pawel Pelczar

   Article

   Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

   Type

   Empty backbone

   Use

   Tags

   FokI nuclease (ELD)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

   Plasmid

   #40131

   Purpose

   Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

   Depositor

   Pawel Pelczar

   Article

   Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

   Type

   Empty backbone

   Use

   Tags

   FokI nuclease (KKR)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pECFP-histone H3

   Plasmid

   #179698

   Purpose

   visualization of nucleus/chromosome

   Depositor

   Kenji Sugimoto

   Article

   Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

   Insert

   Histone H3 (Hist1h3h Mouse)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR4 TNRC6C

   Plasmid

   #20023

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   Trinucleotide repeat containing 6C (TNRC6C Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-T3-eSpCAS9-NG-pA

   Plasmid

   #131466

   Purpose

   Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

   Insert

   Codon optimized eSpCas9-NG
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-T3-SpCAS9-NG-pA

    Plasmid

    #131465

    Purpose

    Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

    Insert

    Codon optimized SpCas9-NG
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15b I-SceI (Ctermhis -MH)

    Plasmid

    #195613

    Purpose

    Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

    Insert

    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57-mcGAS-LL/RK-NLS

    Plasmid

    #185457

    Purpose

    Inducible nuclear cGAS

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    L157R, L159K

    Promoter

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDESTmycTNRC6C

    Plasmid

    #19874

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Trinucleotide repeat containing 6C (TNRC6C Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH6Z-806

    Plasmid

    #85255

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCHNFI-B2

    Plasmid

    #31405

    Depositor

    Richard Gronostajski

    Article

    Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

    Insert

    Nuclear Factor IB isoform 2 (Nfib Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Not the same as reference seq. See sequence for m…

    Promoter

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL8_3xG34-DCAF8L2_X1

    Plasmid

    #191519

    Purpose

    Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE 3xG34-DCAF8L2 X1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pL5_3xG22-DCAF8L2_X1

    Plasmid

    #191516

    Purpose

    Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE 3xG22-DCAF8L2 X1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LDB107

    Plasmid

    #185423

    Purpose

    NUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporin

    Depositor

    Laura Davis, Ken Belanger

    Article

    Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

    Insert

    NUP1
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBzCas13b

    Plasmid

    #164862

    Purpose

    Inducible expression of BzCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    Bergeyella zoohelcum Cas13b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYZ675

    Plasmid

    #132954

    Purpose

    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

    Insert

    dFnCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    nucleoplasmin NLS

    Expression

    Mutation

    Promoter

    adh1

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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