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DGP106- pSP64 Poly(A)1200

Plasmid

#70101

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

1200bp insert from B. subtillis

Use

In vitro transcription

Promoter

SP6

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
CpcBfull-NaGLS fusion_CpcA

Plasmid

#74004

Purpose

Expresses the Nicotiana attenuata GLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Insert

NaGLS and CmR

Promoter

cpc

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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RCAS(A) cTbx5 EnR (CT#635)

Plasmid

#13967

Depositor

Cliff Tabin

Article

Rallis et al Development. 2003 Jun . 130(12):2741-51.

Insert

Tbx5 (TBX5 Chicken)

Use

Retroviral; Avian expression

Mutation

Amino acids 1-274

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
RCAS(A) cTbx5 VP16 (CT#630)

Plasmid

#13969

Depositor

Cliff Tabin

Article

Rallis et al Development. 2003 Jun . 130(12):2741-51.

Insert

Tbx5 (TBX5 Chicken)

Use

Retroviral; Avian expression

Mutation

Amino acids 1-274

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pDonor_Tn[GFP]

Plasmid

#213903

Purpose

Mariner transposon expressing mNeonGreen with kan selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and mNeonGreen

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[RFP]

Plasmid

#213904

Purpose

Mariner transposon expressing mCherry with cam selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and mCherry

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP2373

Plasmid

#160079

Purpose

Z. mobilis CRISPRi, promoter F, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2371

Plasmid

#160078

Purpose

Z. mobilis CRISPRi, promoter E, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2369

Plasmid

#160077

Purpose

Z. mobilis CRISPRi, promoter D, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2132

Plasmid

#160075

Purpose

Z. mobilis CRISPRi, promoter B, empty guide

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2093

Plasmid

#160074

Purpose

Z. mobilis CRISPRi, promoter B, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pME-nls

Plasmid

#108882

Purpose

Multisite gateway vector for 5' tagging with an N-terminal nuclear localisation sequence

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls

Use

Multisite gateway

Available Since

Oct. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pWS1859

Plasmid

#123065

Purpose

Positive regulator of Ste20

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste50 [3]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS511

Plasmid

#123063

Purpose

Negative regulator of Ste12

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Dig2 [3]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS510

Plasmid

#123062

Purpose

Negative regulator of Ste12

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Dig1 [3]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS509

Plasmid

#123061

Purpose

Negative regulator of Fus3

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Msg5 [3]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS081

Plasmid

#123060

Purpose

Regulator of G protein signalling

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Sst2 [3]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS626

Plasmid

#123045

Purpose

P-factor GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Mam2 [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS495

Plasmid

#123043

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pRNR2m [2b]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS497

Plasmid

#123042

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pCUP1m [2b]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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