We narrowed to 8,594 results for: Set

1. GGE038

   Plasmid

   #120785

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_Zeta-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GGE067

   Plasmid

   #120784

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_Zeta-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. BASIC_7_J23101-RBS34-mCherry-B0015

   Plasmid

   #68141

   Purpose

   storage vector for BASIC part: RFP expression cassette

   Depositor

   Geoffrey Baldwin

   Article

   Storch et al ACS Synth Biol. 2015 Apr 16.

   Insert

   J23101-RBS34-mcherry-B0015
   

   Expression

   Bacterial

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAZ-donor

   Plasmid

   #112359

   Purpose

   CRISPR donor plasmid to tag human transcription factor MAZ with GFP

   Depositor

   Kevin White

   Insert

   MAZ homology arms flanking EGFP-IRES-Neo cassette (MAZ Human)
   

   Use

   CRISPR

   Mutation

   hg38:16:29809765:G>A

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Thy1.2-NLS_HA_Dre-P2A-BFP

   Plasmid

   #51275

   Purpose

   Co-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassette

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS_HA_Dre-P2A-BFP
   

   Promoter

   Thy1.2

   Available Since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenticrispr-wt-ltr-puro

   Plasmid

   #173428

   Purpose

   Expresses Cas9 and guide RNA, contains intact LTR

   Depositor

   Jakob Reiser

   Article

   Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

   Insert

   S. pyogenes sgRNA cassette
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiguide-puro-wt-ltr

   Plasmid

   #182635

   Purpose

   Expresses guide RNA, contains intact LTR

   Depositor

   Jakob Reiser

   Article

   Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

   Insert

   S. pyogenes sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. YN2_1_IL50_HOlocus

   Plasmid

   #194426

   Purpose

   Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

   Insert

   Cas9
   

   Expression

   Yeast

   Mutation

   NA

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Myog'-2A-mCherryNLS-PGK-Puro

   Plasmid

   #69548

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm
   Myogenin 3' homology arm (Myog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICSL70002

    Plasmid

    #50333

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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