We narrowed to 721 results for: SG

1. pLAP-BUBR1-D882N

   Plasmid

   #114062

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

   Insert

   BUBR1-D882N (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance), BUBR1-D882N

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLAP-BUBR1-K795R

   Plasmid

   #114060

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

   Insert

   BUBR1-K795R (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance), BUBR1-K795R

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBrain-GFP-TACC3KDP∆682-688-shTACC3

   Plasmid

   #69116

   Purpose

   Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   shTACC3
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 682-688 (region containin…

   Promoter

   CMV

   Available since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRCIscript-crhr1_TAL1B

   Plasmid

   #44235

   Depositor

   Stephen Ekker, Karl Clark

   Article

   Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

   Insert

   crhr1 pTAL scaffold 1B (crhr1 Zebrafish)
   

   Use

   Mrna transcription vector

   Tags

   Expression

   Mutation

   Promoter

   T3

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRCIscript-crhr1_TAL1A

   Plasmid

   #44234

   Depositor

   Stephen Ekker, Karl Clark

   Article

   Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

   Insert

   crhr1 pTAL scaffold 1A (crhr1 Zebrafish)
   

   Use

   Mrna transcription vector

   Tags

   Expression

   Mutation

   Promoter

   T3

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pScalps-Puro-FLAG_HA-Regnase-1 D141N

   Plasmid

   #222663

   Purpose

   Lentiviral vector to express mouse Regnase-1 D141N mutant tagged with FLAG-HA at N-term

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12a (Zc3h12a Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   mutated aspartic acid 141 to asparagine to abroga…

   Promoter

   SFFV

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL4.23-WIPI1promotor

   Plasmid

   #206840

   Purpose

   The plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).

   Depositor

   Tassula Proikas-Cezanne

   Article

   Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

   Insert

   WIPI1 promotor (partial)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141N

   Plasmid

   #222650

   Purpose

   For in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-term

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12a (Zc3h12a Mouse)
   

   Use

   In vitro transcription

   Tags

   FLAG and HA

   Expression

   Mutation

   mutated aspartic acid 141 to asparagine to abroga…

   Promoter

   T7

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGECPL.BC10.MG

   Plasmid

   #223822

   Purpose

   Contains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.

   Depositor

   Dawid Nowak

   Article

   Serio et al Cancer Discov. 2024 Jul 5. doi: 10.1158/

   Insert

   Ef1a-Driven Cre-P2A-FLuc
   

   Use

   CRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-IRES-blast 3xFlag-SPNS1(R76A)

    Plasmid

    #198570

    Purpose

    SPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    changed arginine R76 to alanine (R76A)

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge