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pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WT

Plasmid

#222649

Purpose

For in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Promoter

T7

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-3xFlag-SPNS1

Plasmid

#198568

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag (SPNS1 Human)

Use

Retroviral

Tags

3xFlag

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-Cys-HA-Ubiquitin

Plasmid

#185356

Purpose

In vitro ubiquitination assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Ubiquitin

Tags

GST, HA(x2)

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRSCR

Plasmid

#185345

Purpose

Expression of soluble G2BR with scrambled sequence

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

scrambled gp78 G2BR sequence

Tags

EGFP

Expression

Mammalian

Mutation

scrambled sequence

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2

Plasmid

#185346

Purpose

Membrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Cue1p/Ube2G2 fusion (internal MYC tag)

Expression

Mammalian

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

Plasmid

#184774

Purpose

Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Budding Yeast, Mouse)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_COL4A3_self-cleavingCas9

Plasmid

#130281

Purpose

Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

spCas9

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_COL4A5_self-cleavingCas9

Plasmid

#130282

Purpose

Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

spCas9

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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GoldyTALEN-ppp1cab TAL2

Plasmid

#41836

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

protein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)

Use

Cloning vector

Available Since

Feb. 1, 2013

Availability

Academic Institutions and Nonprofits only

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GoldyTALEN-moesina TAL1

Plasmid

#41831

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

moesin a (msna Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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