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II-ATCC

Plasmid

#43272

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

II-ATCC

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
p2T-CMV-ABE8e_dNRCH-BlastR

Plasmid

#232720

Purpose

A-to-G base editor

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

ABE8e-dCas9(NRCH)

Expression

Mammalian

Mutation

within Cas9 D10A H840A

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHLSec2-irisin-his

Plasmid

#122729

Purpose

Expresses in mammalian cells (HEK293):glycosylated and secreted irisin-his

Depositor

Harold Erickson

Article

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Rat, Human, Mouse, Bovine)

Tags

his and signal sequence for secretion

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-TMEM165

Plasmid

#132272

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

TMEM165 (TMEM165 Human)

Expression

Mammalian

Available Since

Jan. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 ORF3B

Plasmid

#141272

Purpose

Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3B

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1 puro CXCR4 siRNA-2

Plasmid

#12272

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 JN.1.11.1

Plasmid

#223272

Purpose

Encodes SARS-CoV-2 spike JN.1.11.1 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike JN.1.11.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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GPR182-DuET

Plasmid

#213272

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GPR182 (GPR182 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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WT USP7 TRAF

Plasmid

#227226

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Article

Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 62-205

Promoter

T7-promoter

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-GFP-AnillinFL

Plasmid

#187272

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6, CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJC2741 - hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro

Plasmid

#250272

Purpose

Ratiometric protein stability reporter for measuring C-terminal degron activity.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

EGFP-CycD1(C-term)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA T49I

Plasmid

#249272

Purpose

Expression of PKA T49I mutant (PRKACAT49I)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

T49I

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-eGFP

Plasmid

#227237

Purpose

Large capacity AAV that expresses eGFP in neurons expressing Cre recombinase

Depositor

Richard Huganir

Article

Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

Insert

eGFP (eGFP )

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pSS02 NAPstar2

Plasmid

#241272

Purpose

Expresses NADPH/NADP+ biosensor NAPstar2 in Arabidopsis thaliana

Depositor

Markus Schwarzländer

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

cyt-NAPstar2

Expression

Plant

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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WT USP7 UBL-12

Plasmid

#227230

Purpose

Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Article

Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 535-775

Promoter

T7-promoter

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF1082

Plasmid

#142724

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FUBP1 (FUBP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-Repo-Man-1-890

Plasmid

#184272

Purpose

Expresses C-terminally truncated GFP-RepoMan

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Tags

GFP

Expression

Mammalian

Mutation

truncated Repo-Man after aminoacid 893

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-OSCAR-COMP5AP-AviTag-9xHis

Plasmid

#157272

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

OSCAR (OSCAR Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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289aa ELL2

Plasmid

#127266

Purpose

For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Three Mets (133, 138, 186) to Ileu. Contains 289…

Promoter

T7

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Xba ELL2 Met133, 138, 186 to Ileu

Plasmid

#127269

Purpose

For in vitro translation of ELL2 with with internal HA tag and Met133, 138, 186 Ileu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 Met133, 138, 186 to Ileu (ELL2 Human)

Use

In vitro translation

Mutation

Met133, 138, 186 to Ileu HA tag after M186

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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