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We narrowed to 2,467 results for: PUC19

Showing: 1201 - 1220 of 2467 results
  1. pIAeBlue

    Plasmid
    #185891
    Purpose
    In vivo gene amplification (HapAmp) of AeBlue expression module at RPL25 locus
    Depositor
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pISL1-donor

    Plasmid
    #113820
    Purpose
    CRISPR donor plasmid to tag human transcription factor ISL1 with GFP
    Depositor
    Insert
    ISL1 homology arms flanking EGFP-IRES-Neo cassette (ISL1 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pERF-donor

    Plasmid
    #112299
    Purpose
    CRISPR donor plasmid to tag human transcription factor ERF with GFP
    Depositor
    Insert
    ERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)
    Use
    CRISPR
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pShoHELIX-sgRNA_entry (BO3)

    Plasmid
    #181784
    Purpose
    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Insert
    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid
    #181781
    Purpose
    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Insert
    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pTSHZ2-donor

    Plasmid
    #113810
    Purpose
    CRISPR donor plasmid to tag human transcription factor TSHZ2 with GFP
    Depositor
    Insert
    TSHZ2 homology arms flanking EGFP-IRES-Neo cassette (TSHZ2 Human)
    Use
    CRISPR
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. qTAG-N-Solo-mStayGold-TJP1

    Plasmid
    #227300
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)
    Depositor
    Insert
    TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 1201 - 1220 of 2467 results