We narrowed to 9,931 results for: SEC

1. pECIA14

   Plasmid

   #47051

   Purpose

   This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

   Depositor

   Chris Garcia

   Article

   Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

   Type

   Empty backbone

   Use

   Secreted expression in drosophila culture

   Tags

   Alkaline Phosphatase (human, placental), Flag, HR…

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein (Copper-Inducible)

   Available since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCPP5233

   Plasmid

   #128715

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

   Insert

   shcM-hopM1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCPP5060

   Plasmid

   #128716

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

   Insert

   shcN-hopN1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACEBac1-CoRESTfl

   Plasmid

   #109156

   Purpose

   Encodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Inui et al Protein Sci. 2017 May;26(5):997-1011. do

   Insert

   human full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Jan. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 908H

   Plasmid

   #160240

   Purpose

   TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Tet Operator (2x)
   hsp70 minimal promoter
   p-element promoter
   Firefly luciferase
   Renilla luciferase
   Ubiquitin promoter
   

   Use

   Tags

   T2A GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDropIn-T2A-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199195

   Purpose

   Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCPP3373

   Plasmid

   #128718

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopQ1-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Synonymous point mutation in the wobble position…

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC304

   Plasmid

   #118897

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with TEVprotease and TwinStrep-MB…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOCC119

   Plasmid

   #118884

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
   

   Use

   Tags

   HIS6-MBP, cleavable with 3C protease and mGFP, cl…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA

    Plasmid

    #97012

    Purpose

    Rosa26 targeting vector for intersectional Cre and FLP responsive cassette

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. JPF0419d

    Plasmid

    #124020

    Purpose

    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOCC175

    Plasmid

    #118890

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease; mGFP, cleav…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOCC240

    Plasmid

    #118895

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    MBP, cleavable with 3C protease and mCherry, clea…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149426

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[Sxl, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOCC120

    Plasmid

    #118892

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal monomeric GFP and HIS6, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    MBP, cleavable with 3C protease and mGFP, HIS6, c…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFastBac HT JS-Munc18b

    Plasmid

    #135554

    Purpose

    Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18b (Stxbp2 Rat)
    

    Use

    Tags

    6xHis tag and TEV site

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOCC254

    Plasmid

    #118889

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease; mCherry, cl…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 908E

    Plasmid

    #160238

    Purpose

    TtgO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Ttg Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 908G

    Plasmid

    #160239

    Purpose

    PipO(3x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Pip Operator (3x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 908J

    Plasmid

    #160242

    Purpose

    CymO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Cym Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge