Addgene

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pJJB1426

Plasmid

#218622

Purpose

Express tNCS to the peroxisome for toxicity assay

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

tNCS

Use

Tags

YFP-ePTS1

Expression

Yeast

Mutation

106-588

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397C_ABE

Plasmid

#189643

Purpose

16S rRNA Right_G1397C_ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397N

Plasmid

#189644

Purpose

16S rRNA Right_G1397N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

RPS5A

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AatII-pRPS5A-PTP-3Xflag- psa A Right _G1397C_ABE

Plasmid

#189648

Purpose

psa A Right _G1397C_ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 C-ABE

Plasmid

#189650

Purpose

rbc L 294 Left_G1397 C-ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-inducible Luciferase reporter

Plasmid

#64127

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

TetOx13-CMV

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCAMBgfp

Plasmid

#206130

Purpose

This binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.

Depositor

Anne Osbourn

Article

Sesma et al Nature. 2004 Sep 30;431(7008):582-6. doi

Insert

hygromycin resistance gene and the Promoter of ToxA+SGFP

Use

Binary vector for expression of sgfp in filamento…

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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ND4 DdCBE G1

Plasmid

#221978

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ND4 DdCBE C2

Plasmid

#221979

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4 DdCBE T1

Plasmid

#221980

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4 DdCBE T2

Plasmid

#221981

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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YFP-Rac2

Plasmid

#11393

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 2 (RAC2 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pKS235

Plasmid

#191855

Purpose

Negative selection marker for MosSCI

Depositor

K Subramaniam

Article

Revathi et al MicroPubl Biol. 2022 Oct 20;2022:10.1791

Insert

peel-1 toxic protein for negative selection against extrachromosomal array

Use

Tags

Expression

Worm

Mutation

Promoter

hsp-16.41

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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YFP-Rac2(V12)

Plasmid

#11397

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 2 (RAC2 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Glycine 12 changed to Valine.

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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YFP-Rac2(N17)

Plasmid

#11398

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 2 (RAC2 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Threonine 17 changed to Asparagine.

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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YFP-Rac1(N17)

Plasmid

#11395

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Threonine 17 mutated to Asparagine.

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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YFP-Rac1(L61)

Plasmid

#11401

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Glutamine 61 mutated to Leucine.

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
MZB216

Plasmid

#221766

Purpose

"Empty" C-terminal CPD-10xHis vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

rtxA

Use

Tags

Expression

Mutation

Promoter

T7 Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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TC DdCBE A1

Plasmid

#221988

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-TC TALE A1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TC DdCBE T2

Plasmid

#221989

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-TC TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge